![Western Blot: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Total protein from mouse placenta and spleen was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RT1b in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Azide-and-BSA-Free-Western-Blot-NBP2-80849-img0002.jpg "Western Blot: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Total protein from mouse placenta and spleen was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RT1b in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
| Reactivity | Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OX-6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Rat thymocyte membrane glycoproteins |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RT1-B |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. PLP fixation is recommended for optimal results. This antibody is CyTOF ready. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for MHC Class II RT1B Antibody (NBP2-80849)Find related products by research area.
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Read full blog post. |
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The role of MHC Class II RT1B and immune response post brain injury The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells. Once recognized, the foreign pathogen is typically evade... Read full blog post. |
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| Gene Symbol | RT1-B |
![Immunocytochemistry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse spleen using MHC Class II RT1B antibody (clone OX-6) at 1:100 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the nuclei were coun](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Azide-and-BSA-Free-Immunocytochemistry-NBP2-80849-img0003.jpg "Immunocytochemistry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse spleen using MHC Class II RT1B antibody (clone OX-6) at 1:100 dilution. The signal was developed using HRP-labelled secondary antibody and DAB reagent, and the nuclei were coun")
![Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Staining of rat splenocytes with Mouse anti Rat MHC class II RT1B Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80849-img0009.jpg "Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Staining of rat splenocytes with Mouse anti Rat MHC class II RT1B Image from the standard format of this antibody.")
![Western Blot: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Rat Spleen Tissue lysate probed with MHC Class II antibody. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Azide-and-BSA-Free-Western-Blot-NBP2-80849-img0001.jpg "Western Blot: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Rat Spleen Tissue lysate probed with MHC Class II antibody. Image from the standard format of this antibody.")
![Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Analysis using Alexa Fluor (R) 488 conjugate of NB100-65541. Rat Splenocytes were stained with MHC Class II RT1B (OX-6) antibody NB100-65541 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80849-img0004.jpg "Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Analysis using Alexa Fluor (R) 488 conjugate of NB100-65541. Rat Splenocytes were stained with MHC Class II RT1B (OX-6) antibody NB100-65541 (blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. The antibodies were directly conjugated to Alexa Fluor 488.")
![Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Staining of rat spleen cells. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80849-img0005.jpg "Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Staining of rat spleen cells. Image from the standard format of this antibody.")
![Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Staining of rat spleen cells. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80849-img0006.jpg "Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Staining of rat spleen cells. Image from the standard format of this antibody.")
![Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Staining of rat spleen cells. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80849-img0007.jpg "Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Staining of rat spleen cells. Image from the standard format of this antibody.")
![Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Staining of rat spleen cells. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/MHC-Class-II-RT1B-Antibody-OX-6-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80849-img0008.jpg "Flow Cytometry: MHC Class II RT1B Antibody (OX-6) - Azide and BSA Free [NBP2-80849] - Staining of rat spleen cells. Image from the standard format of this antibody.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")