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MHC Class II RT1B Antibody (OX-6) [Janelia Fluor® 635]

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Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready, IHC
Clone
OX-6
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 635

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MHC Class II RT1B Antibody (OX-6) [Janelia Fluor® 635] Summary

Immunogen
Rat thymocyte membrane glycoproteins
Specificity
NB100-65541 recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. This antibody cross reacts with certain mouse strains of MHC haplotypes k and s. This antibody does not react with C57BL6 or C57BL6/j mice because they are haplotype b. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. The clone does not react with Rat BDIX strain. Please be aware of the possible size variance seen in western blot.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RT1-B
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry Free-Floating
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for MHC Class II RT1B Antibody (OX-6) [Janelia Fluor® 635]

  • DMA
  • DMB
  • DP beta 1
  • DPB 1
  • DPB1
  • DRB
  • HLA class II histocompatibility antigen DM beta chain
  • HLA DMB
  • HLA DPB1
  • HLADM
  • HLADP1B
  • Major histocompatibility complex class II DP beta 1
  • Major histocompatibility complex class II
  • MHC class II antigen DMB
  • MHC DPB1
  • RING6
  • RING7

Background

MHC Class II antigens are heterodimers consisting of one alpha chain(31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR 3, ER-TR 2, ER-TR 1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MHC Class II RT1B Antibody (NB100-65541JF635) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RT1-B