Home » MGMT » MGMT Antibodies » MGMT Antibody (OTI4F9) [CoraFluor™ 1]
Novus Biologicals products are now on bio-techne.com

MGMT Antibody (OTI4F9) [CoraFluor™ 1]

 Submit a Review

Images

 
There are currently no images for MGMT Antibody (NBP2-72659CL1).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
OTI4F9
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72659AF350
Alexa Fluor 405
NBP2-72659AF405
Alexa Fluor 488
NBP2-72659AF488
Alexa Fluor 532
NBP2-72659AF532
Alexa Fluor 594
NBP2-72659AF594
Alexa Fluor 647
NBP2-72659AF647
Alexa Fluor 700
NBP2-72659AF700
Alexa Fluor 750
NBP2-72659AF750
Allophycocyanin
NBP2-72659APC
Biotin
NBP2-72659B
DyLight 350
NBP2-72659UV
DyLight 405
NBP2-72659V
DyLight 488
NBP2-72659G
DyLight 550
NBP2-72659R
DyLight 594
NBP2-72659DL594
DyLight 650
NBP2-72659C
DyLight 680
NBP2-72659FR
DyLight 755
NBP2-72659IR
FITC
NBP2-72659F
HRP
NBP2-72659H
Janelia Fluor 525
NBP2-72659JF525
Janelia Fluor 549
NBP2-72659JF549
Janelia Fluor 646
NBP2-72659JF646
Janelia Fluor 669
NBP2-72659JF669
PE
NBP2-72659PE
PerCP
NBP2-72659PCP
Unconjugated
NBP2-45843
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-45843
Azide and BSA Free
NBP2-72659
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

MGMT Antibody (OTI4F9) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Full length human recombinant protein of human MGMT (NP_002403) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MGMT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for MGMT Antibody (OTI4F9) [CoraFluor™ 1]

  • 6-O-methylguanine-DNA methyltransferase
  • EC 2.1.1.63
  • methylated-DNA--protein-cysteine methyltransferase
  • methylguanine-DNA methyltransferase
  • MGMT
  • O-6-methylguanine-DNA methyltransferase
  • O6-methylguanine-DNA methyltransferase
  • O-6-methylguanine-DNA-alkyltransferase

Background

O6-Methylguanine-DNA Methyltransferase (MGMT) is an important DNA repair protein involved in tumor cell resistance to the cytotoxic activity of chemotherapeutic alkylating agents. This protein is also effective in protecting normal cells against the genotoxic and carcinogenic effects of DNA alkylation. The alkylating drug resistance is caused by MGMT's ability to remove DNA alkyl groups introduced in the O6 position of guanine. MGMT is expressed in highly variable amounts, depending upon the cell and tissue type, species, and cellular growth characteristics. In addition, MGMT activity varies among groups of tumors and within a particular type of tumor.

MGMT antibodes are useful tools for DNA repair studies and for some cancer research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

p19ARF/CDKN2A
Antibody - BSA Free
NB200-106
Immunohistochemistry p19ARF/CDKN2A Antibody - BSA Free
Western Blot p19ARF/CDKN2A Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence p19ARF/CDKN2A Antibody - BSA Free
(1 Review)
(48 Publications)
Species: Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
MLH1 Antibody
(SP08-04)
NBP2-67381
Flow Cytometry MLH1 Antibody (SP08-04)
Immunocytochemistry/ Immunofluorescence MLH1 Antibody (SP08-04)
Immunohistochemistry-Paraffin MLH1 Antibody (SP08-04)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, KO, WB
p53 Antibody (PAb
240) - BSA Free
NB200-103
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow Cytometry p53 Antibody (PAb 240) - BSA Free
Flow (Intracellular) p53 Antibody (PAb 240) - BSA Free
(3 Reviews)
(42 Publications)
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
RASSF1 Antibody
(OTI2B11)
NBP2-03644
Flow Cytometry RASSF1 Antibody (OTI2B11)
Western Blot RASSF1 Antibody (OTI2B11)
Immunohistochemistry-Paraffin RASSF1 Antibody (OTI2B11)
(1 Publication)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
E-Cadherin
Antibody [Unconjugate
AF748
Immunocytochemistry E-Cadherin Antibody [Unconjugated]
Immunohistochemistry E-Cadherin Antibody [Unconjugated]
Immunocytochemistry E-Cadherin Antibody [Unconjugated]
(142 Publications)
Species: Hu, Mu
Applications: CyTOF-ready, Dual ISH-IHC, Flow, ICC, IHC, Simple Western, WB
DAP Kinase 1
Antibody (SR1225)
NBP3-21649
Immunohistochemistry DAP Kinase 1 Antibody (SR1225)
Western Blot DAP Kinase 1 Antibody (SR1225)
Immunocytochemistry/ Immunofluorescence DAP Kinase 1 Antibody (SR1225)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, WB
APC Antibody - BSA
Free
NB100-91662
Immunocytochemistry/ Immunofluorescence APC Antibody - BSA Free
Flow Cytometry APC Antibody - BSA Free
Immunohistochemistry-Paraffin APC Antibody - BSA Free
(2 Publications)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
SUB1 Antibody
NBP1-82454
Immunohistochemistry-Paraffin SUB1 Antibody
Immunohistochemistry-Paraffin SUB1 Antibody
Immunohistochemistry-Paraffin SUB1 Antibody
(2 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
DNA Ligase IV
Antibody
NBP2-16182
Western Blot DNA Ligase IV Antibody
Immunohistochemistry-Paraffin DNA Ligase IV Antibody
Immunocytochemistry/ Immunofluorescence DNA Ligase IV Antibody
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
MSP/MST1
[Unconjugated]
352-MS
MSP/MST1 [Unconjugated]
(9 Publications)
Species: Hu
Applications: BA, BA
Glutathione
S-Transferase pi 1/GS ...
NBP2-16756
Western Blot Glutathione S-Transferase pi 1/GSTP1 Antibody
Immunocytochemistry/ Immunofluorescence Glutathione S-Transferase pi 1/GSTP1 Antibody
Immunohistochemistry-Paraffin Glutathione S-Transferase pi 1/GSTP1 Antibody
(1 Review)
(2 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
PSP94/MSMB
Antibody (OTI6C7)
NBP2-02450
Western Blot PSP94/MSMB Antibody (OTI6C7)
Immunocytochemistry/ Immunofluorescence PSP94/MSMB Antibody (OTI6C7)
Immunohistochemistry-Paraffin PSP94/MSMB Antibody (OTI6C7)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Invadolysin
Antibody
NBP1-92025
Western Blot Invadolysin Antibody
Western Blot Invadolysin Antibody
Immunohistochemistry-Paraffin Invadolysin Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Recombinant Human
TMPRSS13 GST (N-Term) ...
H00084000-Q01
SDS-PAGE Recombinant Human TMPRSS13 GST (N-Term) Protein
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
Coagulation Factor
XIV/Protein C Antibod ...
AF4885
Coagulation Factor XIV/Protein C Antibody
(2 Publications)
Species: Mu
Applications: IP, WB
EGFR Antibody
[Unconjugated]
AF231
Immunohistochemistry EGFR Antibody [Unconjugated]
Immunocytochemistry EGFR Antibody [Unconjugated]
Western Blot EGFR Antibody [Unconjugated]
(30 Publications)
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, Simple Western, WB
Isocitrate
Dehydrogenase 1/IDH1 ...
MAB7049
Knockout Validated Isocitrate Dehydrogenase 1/IDH1 Antibody (843219) [Unconjugated]
Immunocytochemistry Isocitrate Dehydrogenase 1/IDH1 Antibody (843219) [Unconjugated]
Western Blot Isocitrate Dehydrogenase 1/IDH1 Antibody (843219) [Unconjugated]
(6 Publications)
Species: Hu
Applications: ICC, IHC, KO, Simple Western, WB
S100A9 Antibody
[Unconjugated]
AF2065
Immunocytochemistry S100A9 Antibody [Unconjugated]
Immunohistochemistry S100A9 Antibody [Unconjugated]
Immunocytochemistry S100A9 Antibody [Unconjugated]
(2 Reviews)
(34 Publications)
Species: Mu
Applications: ICC, IHC, Simple Western, WB
SF3B14 Antibody
NBP1-87431
Immunocytochemistry/ Immunofluorescence SF3B14 Antibody
Immunohistochemistry-Paraffin SF3B14 Antibody
Immunohistochemistry-Paraffin SF3B14 Antibody
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
ROBLD3 Antibody
NBP1-71687
Immunocytochemistry/ Immunofluorescence ROBLD3 Antibody
Immunohistochemistry ROBLD3 Antibody
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P

Publications for MGMT Antibody (NBP2-72659CL1) (0)

There are no publications for MGMT Antibody (NBP2-72659CL1).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MGMT Antibody (NBP2-72659CL1) (0)

There are no reviews for MGMT Antibody (NBP2-72659CL1). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MGMT Antibody (NBP2-72659CL1) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional MGMT Products

Research Areas for MGMT Antibody (NBP2-72659CL1)

Find related products by research area.

Blogs on MGMT

There are no specific blogs for MGMT, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Goat anti-Mouse IgG Secondary Antibody [HRP]
HAF007
SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.
Mouse IgG1 Isotype Control (MG1)
NBP1-97005-0.5mg

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MGMT Antibody (OTI4F9) [CoraFluor™ 1] and receive a gift card or discount.


Bioinformatics

Gene Symbol MGMT