IHC: NB100-80870 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity..
Reviewed Applications
Read 1 Review rated 5 using NB100-80870 in the following applications:
Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Buffer
20mM Potassium Phosphate (pH 7.2) and 0.15M NaCl
Preservative
0.01% Sodium Azide
Concentration
1.0 mg/ml
Purity
Affinity purified
Alternate Names for MDR1/ABCB1 Antibody - BSA Free
ABC20
ABCB1
ABCB1B
ATP-binding cassette sub-family B member 1
ATP-binding cassette, sub-family B (MDR/TAP), member 1
CD243 antigen
CD243
CLCS
colchicin sensitivity
doxorubicin resistance
EC 3.6.3
EC 3.6.3.44
GP170
IBD13
MDR1
MDR1MGC163296
multidrug resistance protein 1
P-glycoprotein 1
P-gp
PGY1
PGY1P-GP
Background
P Glycoprotein, the product of the MDR1 gene, is expressed in distinct non-malignant cells, typically cells with secretory and excretory functions. It is assumed to function as an ATP-dependent drug efflux pump with broad substrate specificity. The highest expression of P Glycoprotein has been observed in kidney (proximal tubules), liver (bile canaliculi), adrenal gland and intestine, suggesting that the primary role of P Glycoprotein is in the normal secretion of physiological metabolites and ingested chemicals into bile, urine and the lumen of the intestinal tract. Elevated levels of P Glycoprotein have also been reported in multidrug-resistant cell lines and in colon, endometrial, ovarian, and breast tumors, as well as in sarcomas and leukemias / lymphomas.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for MDR1/ABCB1 Antibody (NB100-80870). (Showing 1 - 1 of 1 FAQs).
I'm wondering what advice you have in regards to storing antibodies in glycerol solution to prevent freeze thaw damage. I recently purchased an MDR1 antibody, rabbit host and polyclonal. The concentration is 1mg/ml.
Glycerol can indeed be added to the buffer for long term storage to help prevent freeze thaw cycle damage. Our lab recommends adding 30%.
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