| Reactivity | MuSpecies Glossary |
| Applications | PAGE, Enzyme Activity |
| Concentration | 1 mg/ml |
| Description | A recombinant protein with a N-terminalHis-tag and corresponding to the amino acids 25-338 of Mouse MDH2. Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET RANVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMVCIIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL ATLTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVQSKET ECTYFSTPLL LGKKGLEKNL GIGKITPFEE KMIAEAIPEL KASIKKGEDF VKNMK |
| Details of Functionality | Specific activity is > 800 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C |
| Source | E. coli |
| Protein/Peptide Type | Recombinant Protein |
| Gene | MDH2 |
| Purity | >95%, by SDS-PAGE |
| Dilutions |
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| Theoretical MW | 35.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 20% glycerol |
| Preservative | No Preservative |
| Concentration | 1 mg/ml |
| Purity | >95%, by SDS-PAGE |
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | MDH2 |
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