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MBP Antibody (CL2829) - Azide and BSA Free

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Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Mouse Cerebral Cortex tissue
Orthogonal Strategies: Analysis in human cerebral cortex and liver tissues using NBP3-43793 antibody. Corresponding MBP RNA-seq data are presented for the same tissues.
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue
Staining of human cerebral cortex shows strong positivity in myelinated neural fibers.
Staining of human cerebellum shows strong positivity in myelinated neural fibers.
Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Staining of human liver shows no positivity in hepatocytes as expected.
Staining of rat basal forebrain shows strong positivity in myelinated neural fibers.
Staining of mouse cerebellum shows strong positivity in myelinated neural fibers.
Staining of rat cerebral cortex shows strong positivity in myelinated neural fibers.
Staining of mouse hippocampus shows strong positivity in myelinated neural fibers.
Staining of rat cerebral cortex shows strong immunoreactivity in myelinated fibers.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
CL2829
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH
Validated by:
       

Orthogonal Strategies

 

Order Details

MBP Antibody (CL2829) - Azide and BSA Free Summary

Immunogen
This antibody was generated using a recombinant protein sequence of P02686, with the exact immunogen sequence remaining proprietary.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MBP
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized in 5% Trehalose.
Preservative
0.02% Sodium Azide
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for MBP Antibody (CL2829) - Azide and BSA Free

  • Hmbpr
  • MBP
  • MGC99675
  • mld
  • Myelin A1 protein
  • myelin basic protein
  • Myelin membrane encephalitogenic protein
  • shi

Background

Myelin Basic Protein (MBP) functions in the nervous system in myelination and is expressed in oligodendrocytes following differentiation. There are numerous isoforms generated by differential splicing events and post-translational modifications that have specialized functions. These functions include participation in signaling pathways prior to myelination, myelination or the re-myelination process following neural injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MBP