![Western Blot: MAG/Siglec-4a Antibody [NBP2-17201] - Various whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAG antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-Western-Blot-NBP2-17201-img0003.jpg "Western Blot: MAG/Siglec-4a Antibody [NBP2-17201] - Various whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAG antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MAG/Siglec-4a. The exact sequence is proprietary. |
| Localization | Membrane; Single-pass type I membrane protein |
| Predicted Species | Bovine (92%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MAG |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 69 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
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| Publication using NBP2-17201 | Applications | Species |
|---|---|---|
| Borenäs M, Umapathy G, Lind D et al. ALK signalling primes the DNA damage response sensitizing ALK-driven neuroblastoma to therapeutic ATR inhibition bioRxiv 2023-09-04 (IHC, Mouse) | IHC | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for MAG/Siglec-4a Antibody (NBP2-17201)Find related products by research area.
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![Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody [NBP2-17201] - Rat fore brain. MAG antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-Immunohistochemistry-Paraffin-NBP2-17201-img0006.jpg "Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody [NBP2-17201] - Rat fore brain. MAG antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: MAG/Siglec-4a Antibody [NBP2-17201] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MAG antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-Western-Blot-NBP2-17201-img0001.jpg "Western Blot: MAG/Siglec-4a Antibody [NBP2-17201] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MAG antibody diluted at 1:1000.")
![Immunohistochemistry-Frozen: MAG/Siglec-4a Antibody [NBP2-17201] - Frozen-sectioned adult mouse cerebellum. Green: MAG protein stained by MAG antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-Immunohistochemistry-Frozen-NBP2-17201-img0005.jpg "Immunohistochemistry-Frozen: MAG/Siglec-4a Antibody [NBP2-17201] - Frozen-sectioned adult mouse cerebellum. Green: MAG protein stained by MAG antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500.Blue: Fluoroshield with DAPI .")
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![N/A BDNF [HRP]](https://images.novusbio.com/images2/DATA_BDNF_DBD00_ELISA_588.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")