![Western Blot: LMX1A Antibody [NBP2-41193] - Western blot analysis of LMX1A in rat brain tissue lysate with LMX1A antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/LMX1A-Antibody-Western-Blot-NBP2-41193-img0001.jpg "Western Blot: LMX1A Antibody [NBP2-41193] - Western blot analysis of LMX1A in rat brain tissue lysate with LMX1A antibody at (A) 1 and (B) 2 ug/mL.")
| Reactivity | Hu, Mu, Rt, ChSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1 mg/ml |
| Immunogen | Antibody was raised against a 16 amino acid peptide near the carboxy terminus of human LMX1A. The immunogen is located within amino acids 300 - 350 of LMX1A. Amino Acid Squence: CPQMPGDHMHPYGAEPL |
| Specificity | At least two isoforms are known to exist. This antibody is predicted to not cross-react with LMX1B. |
| Predicted Species | Chicken (92%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | LMX1A |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | LMX1A |
![Immunocytochemistry/Immunofluorescence: LMX1A Antibody [NBP2-41193] - Staining of LMX1A in mouse brain tissue with LMX1A Antibodyat 20 ug/mL.](http://images.novusbio.com/fullsize/LMX1A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-41193-img0006.jpg "Immunocytochemistry/Immunofluorescence: LMX1A Antibody [NBP2-41193] - Staining of LMX1A in mouse brain tissue with LMX1A Antibodyat 20 ug/mL.")
![Immunohistochemistry: LMX1A Antibody [NBP2-41193] - Staining of LMX1A in mouse brain tissue with LMX1A Antibodyat 5 ug/mL.](http://images.novusbio.com/fullsize/LMX1A-Antibody-Immunohistochemistry-NBP2-41193-img0007.jpg "Immunohistochemistry: LMX1A Antibody [NBP2-41193] - Staining of LMX1A in mouse brain tissue with LMX1A Antibodyat 5 ug/mL.")
![Immunocytochemistry/Immunofluorescence: LMX1A Antibody [NBP2-41193] - Immunofluorescence of LMX1A in human brain tissue with LMX1A antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/LMX1A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-41193-img0003.jpg "Immunocytochemistry/Immunofluorescence: LMX1A Antibody [NBP2-41193] - Immunofluorescence of LMX1A in human brain tissue with LMX1A antibody at 20 ug/mL.")
![Immunohistochemistry: LMX1A Antibody [NBP2-41193] - Immunohistochemistry of LMX1A in human brain tissue with LMX1A antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/LMX1A-Antibody-Immunohistochemistry-NBP2-41193-img0002.jpg "Immunohistochemistry: LMX1A Antibody [NBP2-41193] - Immunohistochemistry of LMX1A in human brain tissue with LMX1A antibody at 2.5 ug/mL.")
![Western Blot: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 4 Western Blot Validation in Rat Skeletal Muscle Loading: 10 u of lysates per lane.Antibodies: LMX1A, , 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-41193_rabbit-polyclonal-lmx1a-antibody-2711202420441155.gif "Western Blot: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 4 Western Blot Validation in Rat Skeletal Muscle Loading: 10 u of lysates per lane.Antibodies: LMX1A, , 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunocytochemistry/ Immunofluorescence: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 1 Immunofluorescence Validation of LMX1A in HeLa CellsImmunofluorescent analysis of methanol-fixed HeLa cells labeling LMX1A with at 10 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp2-41193_rabbit-polyclonal-lmx1a-antibody-271120242042543.jpg "Immunocytochemistry/ Immunofluorescence: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 1 Immunofluorescence Validation of LMX1A in HeLa CellsImmunofluorescent analysis of methanol-fixed HeLa cells labeling LMX1A with at 10 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.")
![Immunohistochemistry: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 6 Immunohistochemistry Validation of LMX1A in Mouse Brain Tissue Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- anti-LMX1A antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4#730;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp2-41193_rabbit-polyclonal-lmx1a-antibody-2711202419251596.jpg "Immunohistochemistry: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 6 Immunohistochemistry Validation of LMX1A in Mouse Brain Tissue Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- anti-LMX1A antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4#730;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Western Blot: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 2 Western Blot Validation in Human Skeletal Muscle Loading: 10 u of lysates per lane.Au ntibodies: LMX1A, , 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-41193_rabbit-polyclonal-lmx1a-antibody-2711202420115527.gif "Western Blot: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 2 Western Blot Validation in Human Skeletal Muscle Loading: 10 u of lysates per lane.Au ntibodies: LMX1A, , 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 3 Western Blot Validation in Mouse Skeletal Muscle Loading: 10 u of lysates per lane.Antibodies: LMX1A, , 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-41193_rabbit-polyclonal-lmx1a-antibody-2711202420352451.gif "Western Blot: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 3 Western Blot Validation in Mouse Skeletal Muscle Loading: 10 u of lysates per lane.Antibodies: LMX1A, , 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunohistochemistry: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 6 Immunohistochemistry Validation of LMX1A in Mouse Brain Tissue Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- anti-LMX1A antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4#730;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp2-41193_rabbit-polyclonal-lmx1a-antibody-271120242027249.jpg "Immunohistochemistry: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 6 Immunohistochemistry Validation of LMX1A in Mouse Brain Tissue Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- anti-LMX1A antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4#730;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Western Blot: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 4 Western Blot Validation in Rat Skeletal Muscle Loading: 10 u of lysates per lane.Antibodies: LMX1A, , 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-41193_rabbit-polyclonal-lmx1a-antibody-271120241923632.gif "Western Blot: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 4 Western Blot Validation in Rat Skeletal Muscle Loading: 10 u of lysates per lane.Antibodies: LMX1A, , 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunohistochemistry: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 7 Immunohistochemistry Validation of LMX1A in Rat Skeletal Muscle Tissue Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-LMX1A antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp2-41193_rabbit-polyclonal-lmx1a-antibody-2711202420171793.jpg "Immunohistochemistry: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 7 Immunohistochemistry Validation of LMX1A in Rat Skeletal Muscle Tissue Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-LMX1A antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Western Blot: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 2 Western Blot Validation in Human Skeletal Muscle Loading: 10 u of lysates per lane.Au ntibodies: LMX1A, , 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-41193_rabbit-polyclonal-lmx1a-antibody-271120241923691.gif "Western Blot: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 2 Western Blot Validation in Human Skeletal Muscle Loading: 10 u of lysates per lane.Au ntibodies: LMX1A, , 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Western Blot: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 3 Western Blot Validation in Mouse Skeletal Muscle Loading: 10 u of lysates per lane.Antibodies: LMX1A, , 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-41193_rabbit-polyclonal-lmx1a-antibody-2711202419381730.gif "Western Blot: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 3 Western Blot Validation in Mouse Skeletal Muscle Loading: 10 u of lysates per lane.Antibodies: LMX1A, , 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunocytochemistry/ Immunofluorescence: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 1 Immunofluorescence Validation of LMX1A in HeLa CellsImmunofluorescent analysis of methanol-fixed HeLa cells labeling LMX1A with at 10 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp2-41193_rabbit-polyclonal-lmx1a-antibody-2711202420441147.jpg "Immunocytochemistry/ Immunofluorescence: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 1 Immunofluorescence Validation of LMX1A in HeLa CellsImmunofluorescent analysis of methanol-fixed HeLa cells labeling LMX1A with at 10 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.")
![Immunohistochemistry: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 5 Immunohistochemistry Validation of LMX1A in Human Brain Meningioma Immunohistochemical analysis of paraffin-embedded human brain meningioma using anti-LMX1A antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4#730;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp2-41193_rabbit-polyclonal-lmx1a-antibody-2711202420115576.jpg "Immunohistochemistry: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 5 Immunohistochemistry Validation of LMX1A in Human Brain Meningioma Immunohistochemical analysis of paraffin-embedded human brain meningioma using anti-LMX1A antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4#730;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunohistochemistry: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 5 Immunohistochemistry Validation of LMX1A in Human Brain Meningioma Immunohistochemical analysis of paraffin-embedded human brain meningioma using anti-LMX1A antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4#730;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp2-41193_rabbit-polyclonal-lmx1a-antibody-2711202420333875.jpg "Immunohistochemistry: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 5 Immunohistochemistry Validation of LMX1A in Human Brain Meningioma Immunohistochemical analysis of paraffin-embedded human brain meningioma using anti-LMX1A antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4#730;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunohistochemistry: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 7 Immunohistochemistry Validation of LMX1A in Rat Skeletal Muscle Tissue Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-LMX1A antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp2-41193_rabbit-polyclonal-lmx1a-antibody-2711202420352452.jpg "Immunohistochemistry: LMX1A Antibody - BSA Free [NBP2-41193] - Figure 7 Immunohistochemistry Validation of LMX1A in Rat Skeletal Muscle Tissue Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-LMX1A antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
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![Cell Culture FGF-8 [Unconjugated]](https://images.novusbio.com/images/423-f8_recombinant-human-mouse-fgf-8b-protein-cell-culture-1052024145159..jpg)
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![Chromatin Immunoprecipitation (ChIP) HNF-3 beta/FoxA2 Antibody [Unconjugated]](https://images.novusbio.com/images2/HNF-3_beta_AF2400_Chromatin_Immunoprecipitation_11227.jpg)
![Immunocytochemistry HNF-3 beta/FoxA2 Antibody [Unconjugated]](https://images.novusbio.com/images2/HNF-3_beta_AF2400_Immunocytochemistry_6484.jpg)
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![Immunocytochemistry Nurr1/NGFI-B beta/NR4A2 Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af2156_human-mouse-nurr1-ngfi-b-beta-nr4a2-affinity-purified-pab-immunocytochemistry-22112021145420.jpg)
![Immunohistochemistry Nurr1/NGFI-B beta/NR4A2 Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af2156_human-mouse-nurr1-ngfi-b-beta-nr4a2-affinity-purified-pab-immunohistochemistry-10122021111228.jpg)
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![Immunohistochemistry Neurogenin-2 Antibody (7G4) [Unconjugated]](https://images.novusbio.com/images/antibody/mab3314_human-rat-neurogenin-2-mab-clone-7g4-immunohistochemistry-285202411531..jpg)
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![Immunocytochemistry Otx2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Otx2_AF1979_Immunocytochemistry_11033.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")