Novus Biologicals products are now on bio-techne.com

LMO2 Antibody (LMO2/1971) - Azide and BSA Free

Images

 
Western Blot: LMO2 Antibody (LMO2/1971) - Azide and BSA Free [NBP3-08268] - Western Blot Analysis of Ramos cell lysate using LMO2 Mouse Monoclonal Antibody (LMO2/1971).
Immunohistochemistry-Paraffin: LMO2 Antibody (LMO2/1971) - Azide and BSA Free [NBP3-08268] - Formalin-fixed, paraffin-embedded human Pancreas stained with LMO2 Mouse Monoclonal Antibody (LMO2/1971).
Flow Cytometry: LMO2 Antibody (LMO2/1971) - Azide and BSA Free [NBP3-08268] - Flow Cytometric Analysis of K562 cells using LMO2 Mouse Monoclonal Antibody (LMO2/1971) followed by goat anti- Mouse IgG-CF488 (Blue); ...read more
Immunohistochemistry-Paraffin: LMO2 Antibody (LMO2/1971) - Azide and BSA Free [NBP3-08268] - Formalin-fixed, paraffin-embedded human Follicular Lymphoma stained with LMO2 Mouse Monoclonal Antibody (LMO2/1971).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC
Clone
LMO2/1971
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

LMO2 Antibody (LMO2/1971) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07249).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Immunogen
Recombinant human LMO2 protein fragment (around aa 23-140) (exact sequence is proprietary) (Uniprot: P25791)
Localization
Nuclear, Cytoplasmic & Cell Surface
Marker
B-Cell Marker
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
LMO2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Western Blot 1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Others not known.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for LMO2 Antibody (LMO2/1971) - Azide and BSA Free

  • Cysteine-rich protein TTG-2
  • LIM domain only 2 (rhombotin-like 1)
  • LIM domain only protein 2
  • LMO2
  • LMO-2
  • RBTN2
  • RBTN2rhombotin-2
  • RBTNL1
  • RBTNL1rhombotin-like 1
  • RHOM2
  • RHOM2T-cell translocation protein 2
  • T-cell translocation gene 2
  • TTG2
  • TTG2rhombotin 2

Background

LMO2 is a highly conserved oncoprotein that is expressed in human normal germinal-center B cells and in B-cell lymphomas. LMO2 also has a crucial role in hematopoietic development by being necessary for yolk sac erythropoiesis. LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number of T-cell acute lymphoblastic leukemia-specific translocations occur.

LMO2 antibodies are useful tools for erythropoiesis research and certian cancer studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-01488
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NBP1-47492
Species: Pm, Hu
Applications: ChIP, ELISA, ICC/IF, IHC, IHC-P, WB
NBP2-48693
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-47940
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC/IF, IF, IHC, IHC-P, Simple Western, WB
NBP1-31362
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, KO, WB
H00004004-M01
Species: Hu
Applications: ELISA, ICC/IF, WB
NBP3-23662
Species: Hu
Applications: Flow, ICC/IF, IHC-P
MAB7650
Species: Mu
Applications: IHC, WB
AF2046
Species: Hu, Mu
Applications: ICC, IHC, Simple Western, WB
AF6116
Species: Hu
Applications: ICC, WB
NBP1-84843
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-27163
Species: Bv, Ca, Eq, Hu, Mu, Pm
Applications: Flow, WB
NB100-56424
Species: Hu, Mu, Rt
Applications: WB
AF2046
Species: Hu, Mu
Applications: ICC, IHC, Simple Western, WB
NBP2-49342
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-89105
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-80833
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-84841
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-06983
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, PEP-ELISA, WB
NBP3-08268
Species: Hu
Applications: WB, Flow, IHC

Publications for LMO2 Antibody (NBP3-08268) (0)

There are no publications for LMO2 Antibody (NBP3-08268).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for LMO2 Antibody (NBP3-08268) (0)

There are no reviews for LMO2 Antibody (NBP3-08268). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for LMO2 Antibody (NBP3-08268) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional LMO2 Products

Research Areas for LMO2 Antibody (NBP3-08268)

Find related products by research area.

Blogs on LMO2

There are no specific blogs for LMO2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our LMO2 Antibody (LMO2/1971) - Azide and BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol LMO2