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LMAN1 Antibody

Images

 
Western Blot: LMAN1 Antibody [NBP2-19366] - Sample (30 ug of whole cell lysate) A: Hela 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: LMAN1 Antibody [NBP2-19366] - LMAN1 antibody [N1C1] detects LMAN1 protein at cytosol on gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded gastric carcinoma. ...read more
Immunohistochemistry-Paraffin: LMAN1 Antibody [NBP2-19366] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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LMAN1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human LMAN1. The exact sequence is proprietary.
Localization
Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein; Golgi apparatus membrane; Single-pass membrane protein; Endoplasmic reticulum membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LMAN1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (81%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for LMAN1 Antibody

  • ERGIC-53
  • ERGIC53ER-Golgi intermediate compartment 53 kDa protein
  • F5F8Dendoplasmic reticulum-golgi intermediate compartment protein 53
  • FMFD1
  • Gp58
  • intracellular mannose specific lectin
  • Intracellular mannose-specific lectin MR60
  • Lectin mannose-binding 1
  • lectin, mannose-binding, 1
  • MCFD1coagulation factor V-factor VIII combined deficiency
  • MR60
  • protein ERGIC-53

Background

The protein encoded by this gene is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LMAN1
Uniprot