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LIN-28A Antibody [HRP]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
HRP

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-77383AF350
Alexa Fluor 405
NBP1-77383AF405
Alexa Fluor 488
NBP1-77383AF488
Alexa Fluor 532
NBP1-77383AF532
Alexa Fluor 594
NBP1-77383AF594
Alexa Fluor 647
NBP1-77383AF647
Alexa Fluor 700
NBP1-77383AF700
Alexa Fluor 750
NBP1-77383AF750
Biotin
NBP1-77383B
CoraFluor 1
NBP1-77383CL1
DyLight 350
NBP1-77383UV
DyLight 405
NBP1-77383V
DyLight 488
NBP1-77383G
DyLight 550
NBP1-77383R
DyLight 594
NBP1-77383DL594
DyLight 650
NBP1-77383C
DyLight 680
NBP1-77383FR
DyLight 755
NBP1-77383IR
FITC
NBP1-77383F
Janelia Fluor 525
NBP1-77383JF525
Janelia Fluor 549
NBP1-77383JF549
Janelia Fluor 646
NBP1-77383JF646
Janelia Fluor 669
NBP1-77383JF669
PerCP
NBP1-77383PCP
Unconjugated
NBP1-77383
mFluor Violet 450 SE
NBP1-77383MFV450
mFluor Violet 500 SE
NBP1-77383MFV500
mFluor Violet 610 SE
NBP1-77383MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-77383
BSA Free
NBP1-77383
BSA Free
NBP1-77383-0.025mg
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LIN-28A Antibody [HRP] Summary

Immunogen
Antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human Lin28. The immunogen is located within the last 50 amino acids of Lin28. Amino Acid Squence: GPSAQGKPTYFREEE
Marker
Undifferentiated human embryonic stem cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LIN28A
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%)

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
No Preservative
Purity
Peptide affinity purified

Alternate Names for LIN-28A Antibody [HRP]

  • CSDD1
  • CSDD1lin-28A
  • FLJ12457
  • lin-28 homolog (C. elegans)
  • lin-28 homolog A (C. elegans)
  • LIN28
  • LIN-28
  • LIN28A
  • LIN-28A
  • LIN28RNA-binding protein LIN-28
  • Tex17
  • ZCCHC1
  • ZCCHC1protein lin-28 homolog A
  • Zinc finger CCHC domain-containing protein 1
  • zinc finger, CCHC domain containing 1

Background

lin-28 homolog (C. elegans), also known as CSDD1, ZCCHC1. Entrez Protein NP_078950. LIN28 was first discovered in the nematode C. elegans. It is a heterochronic protein in C. elegans involved in the timing of developmental events and choice of stage specific cell fates. LIN28 expression has been found to be regulated post-transcriptionally by miRNAs in both nematodes and mammals. In humans it is expressed in embryonic stem cells and its expression decreases during differentiation. It is negatively regulated by retinoic acid in neuronal differentiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Deriving neural precursor cells from human induced pluripotent stem cells
By Jennifer Sokolowski, MD, PhD.Human induced pluripotent stem cells (iPSCs) can be used to create models of human organ systems and are useful for a) ascertaining the mechanisms underlying pathological conditions...  Read full blog post.
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Bioinformatics

Gene Symbol LIN28A