![Immunohistochemistry: LIM1 Antibody - BSA Free [NB110-12933] - Staining using LIM1 antibody - BSA Free](http://images.novusbio.com/fullsize/LIM1-Antibody---BSA-Free-Immunohistochemistry-NB110-12933-img0001.jpg "Immunohistochemistry: LIM1 Antibody - BSA Free [NB110-12933] - Staining using LIM1 antibody - BSA Free")
| Reactivity | Hu, Mu, Rt, Am, FiSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | For NB110-12933 specific protocl, please see the PDF under the Product PDFs. |
| Immunogen | Synthetic peptide corresponding to C-terminal portion of frog Lim-1. |
| Localization | Nuclear |
| Specificity | Does not appear to crossreact with LIM5 (the closest homologue of LIM1 in frog) on tissue sections but does crossreact with LIM5 in Western blot and immunoprecipitation. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | LHX1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Paraffin): 1:200-1:1000 (Fish and whole mounts: 1:500) Recommended fixative MEMFA, Immunocytochemistry: 1:500 on P19 cell line. Optimal dilutions to be determined by the researcher. Do not use tissues fixed overnight in PFA, the antibody will not work. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02M PBS, pH 7.6 |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB110-12933 | Applications | Species |
|---|---|---|
| Bethea M, Liu Y, Wade AK et al. The islet-expressed Lhx1 transcription factor interacts with Islet-1 and contributes to glucose homeostasis. Am. J. Physiol. Endocrinol. Metab. 2019-01-08 [PMID: 30620636] (ICC/IF, Mouse) | ICC/IF | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for LIM1 Antibody (NB110-12933)Find related products by research area.
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