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LIM Kinase 1 Antibody (1A8)

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Western Blot: LIM Kinase 1 Antibody (1A8) [H00003984-M01] - LIMK1 monoclonal antibody (M01), clone 1A8. Analysis of LIMK1 expression in NIH/3T3.
Immunohistochemistry-Paraffin: LIM Kinase 1 Antibody (1A8) [H00003984-M01] - Analysis of monoclonal antibody to LIMK1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.
Western Blot: LIM Kinase 1 Antibody (1A8) [H00003984-M01] - LIMK1 monoclonal antibody (M01), clone 1A8. Analysis of LIMK1 expression in PC-12.
Western Blot: LIM Kinase 1 Antibody (1A8) [H00003984-M01] - LIMK1 monoclonal antibody (M01), clone 1A8 Analysis of LIMK1 expression in HepG2.
Western Blot: LIM Kinase 1 Antibody (1A8) [H00003984-M01] - LIMK1 monoclonal antibody (M01), clone 1A8. Analysis of LIMK1 expression in SW-13.
Western Blot: LIM Kinase 1 Antibody (1A8) [H00003984-M01] - LIMK1 monoclonal antibody (M01), clone 1A8. Analysis of LIMK1 expression in Raw 264.7.
Sandwich ELISA: LIM Kinase 1 Antibody (1A8) [H00003984-M01] - Detection limit for recombinant GST tagged LIMK1 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, IHC
Clone
1A8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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LIM Kinase 1 Antibody (1A8) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
LIMK1 (NP_002305, 548 a.a. ~ 647 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ADPDYLPRTMDFGLNVRGFLDRYCPPNCPPSFFPITVRCCDLDPEKRPSFVKLEHWLETLRMHLAGHLPLGPQLEQLDRGFWETYRRGESGLPAHPEVPD
Localization
Cytoplasmic
Specificity
LIMK1 - LIM domain kinase 1
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
LIMK1
Purity
Ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Sandwich ELISA 1:100-1:2000
  • Western Blot 1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for LIM Kinase 1 Antibody (1A8)

  • EC 2.7.11.1
  • LIM domain kinase 1
  • LIM Kinase 1
  • LIM motif-containing protein kinase
  • LIMK
  • LIMK1
  • LIMK-1
  • LIMKLIMK-1

Background

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LIMK1
Entrez
OMIM
Uniprot