![Western Blot: SSH1 [p Ser978] Antibody [NBP3-23411] - Western blot of human recombinant SSH1 untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) and rat PC12 cells unstimulated (lanes 5 & 7) or stimulated with calyculin A (lanes 6 & 8). The blots were probed with anti-SSH1 (C-term.) (lanes 1, 2, 5, & 6) or anti-SSH1 (Ser-978) (lanes 3, 4, 7, & 8).](http://images.novusbio.com/fullsize/nbp3-23411_rabbit-ssh1-p-ser978-pab-19920231820293.jpg "Western Blot: SSH1 [p Ser978] Antibody [NBP3-23411] - Western blot of human recombinant SSH1 untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) and rat PC12 cells unstimulated (lanes 5 & 7) or stimulated with calyculin A (lanes 6 & 8). The blots were probed with anti-SSH1 (C-term.) (lanes 1, 2, 5, & 6) or anti-SSH1 (Ser-978) (lanes 3, 4, 7, & 8).")
| Reactivity | Hu, Mu, Rt, ChSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Stable for at least 1 year at -20°C. |
| Immunogen | SSH1 (Ser-978) antibody was generated from a phospho-peptide that included amino acids surrounding serine 978 in human SSH1. This sequence has high homology to the conserved site in rat, mouse, chicken, and zebrafish SSH1L. The sequence also has high homology to the conserved site in SSH2L (Ser-1259). [UniProt# Q8WYL5] |
| Modification | p Ser978 |
| Specificity | The antibody detects a 150 kDa full length recombinant human SSH1 phosphoprotein and this reactivity is eliminated with lambda phosphatase treatment. The antibody also detects SSH1 on immunoblots of rat PC12 and mouse C2C12 cells that were stimulated with calyculin A. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SSH1 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 150 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. |
| Buffer | PBS, 1 mg/ml BSA and 50% glycerol |
| Preservative | 0.05% Sodium Azide |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | SSH1 |
![Immunocytochemistry/Immunofluorescence: SSH1 [p Ser978] Antibody [NBP3-23411] - Immunocytochemical labeling of Slingshot-1L in rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-SSH1 (C-term.) and anti-SSH1 (Ser-978) antibodies, then detected using appropriate secondary antibody conjugated to Cy3 (Right panel). Phase image of corresponding PC12 cells (Left panel).](http://images.novusbio.com/fullsize/nbp3-23411_rabbit-ssh1-p-ser978-pab-19920231240373.jpg "Immunocytochemistry/Immunofluorescence: SSH1 [p Ser978] Antibody [NBP3-23411] - Immunocytochemical labeling of Slingshot-1L in rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-SSH1 (C-term.) and anti-SSH1 (Ser-978) antibodies, then detected using appropriate secondary antibody conjugated to Cy3 (Right panel). Phase image of corresponding PC12 cells (Left panel).")
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