![Immunohistochemistry: Lgr6 Antibody (15G3) [NBP3-26169] - Image of Lgr6 Antibody (15G3) diluted at 1:50 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.26% DAB.](http://images.novusbio.com/fullsize/nbp3-26169_rabbit-lgr6-mab-15g3-262202414471575.jpg "Immunohistochemistry: Lgr6 Antibody (15G3) [NBP3-26169] - Image of Lgr6 Antibody (15G3) diluted at 1:50 and staining in paraffin-embedded human lung cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.26% DAB.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, Flow, ICC/IF, IHC |
| Clone | 15G3 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Additional Information | Recombinant monoclonal antibody expressed in HEK293F cells |
| Immunogen | A synthesized peptide derived from Human Lgr6 [UniProt Q9HBX8] |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | LGR6 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at -20 to -70C. Avoid freeze-thaw cycles. |
| Buffer | PBS, pH 7.4, 150mM NaCl, and 50% glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Lgr6 Antibody (NBP3-26169)Find related products by research area.
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| Gene Symbol | LGR6 |
![Immunocytochemistry/Immunofluorescence: Lgr6 Antibody (15G3) [NBP3-26169] - Staining of hela with Lgr6 Antibody (15G3) at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 500-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26169_rabbit-lgr6-mab-15g3-28220241312228.jpg "Immunocytochemistry/Immunofluorescence: Lgr6 Antibody (15G3) [NBP3-26169] - Staining of hela with Lgr6 Antibody (15G3) at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 500-conjugated Goat Anti-Rabbit IgG (H+L).")
![Flow Cytometry: Lgr6 Antibody (15G3) [NBP3-26169] - Overlay Peak curve showing Hela cells stained with Lgr6 Antibody (15G3) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26169_rabbit-lgr6-mab-15g3-282202414183610.jpg "Flow Cytometry: Lgr6 Antibody (15G3) [NBP3-26169] - Overlay Peak curve showing Hela cells stained with Lgr6 Antibody (15G3) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.")
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