Lgl1 Antibody Summary
Immunogen |
This antibody was developed against Recombinant Protein corresponding to amino acids: FERFSLSARNITEPLCSLDINWPRDATQASYRIRESPKLSQANGTPSILLAPQSLDGSPDPAHSMGPDTPEPPEAALSPMSIDSATSADTTLDTTGDVTVEDVKDFLGSSEESEKNLRNLAEDE |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
LLGL1 |
Purity |
Immunogen affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunohistochemistry 1:200 - 1:500
- Immunohistochemistry-Paraffin 1:200-1:500
- Western Blot 0.04-0.4 ug/ml
|
Application Notes |
For IHC-Paraffin, HIER pH 6 retrieval is recommended. |
Theoretical MW |
115 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Control Peptide |
|
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS (pH 7.2) and 40% Glycerol |
Preservative |
0.02% Sodium Azide |
Purity |
Immunogen affinity purified |
Alternate Names for Lgl1 Antibody
Background
Lgl1 encodes a protein that is similar to a tumor suppressor in Drosophila. The protein is part of a cytoskeletal network and is associated with nonmuscle myosin II heavy chain and a kinase that specifically phosphorylates this protein at serine residues. The gene is located within the Smith-Magenis syndrome region on chromosome 17.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Publications for Lgl1 Antibody (NBP1-84077) (0)
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Product General Protocols
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Video Protocols
FAQs for Lgl1 Antibody (NBP1-84077). (Showing 1 - 1 of 1 FAQ).
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I am interesting in using to analyze LLGL1 sub-cellular localization in my model system. The organotypic model I use is to culture canine kidney cell lines in 3D matrix - the cells form into polarized, cyst structures over time. I treat these cysts with growth factors, to monitor morphogenesis of epithelial tissue. The organoids will be fixed, in PFA, and I will be performing IF with the antibody, since there are currently no LLGL1 antibodies available that detect endogenous protein in canine cell lines. Does this application meet requirements for the Innovators Reward initiative? If our customer is eligible do they have to purchase the antibody at the full price?
- That should be just fine for our Innovators Reward Program. As you know, the customer has to purchase the antibody at full price, and then email innovators@novusbio.com to receive the form to complete and share their data before the Innovator's Reward can be applied.
Secondary Antibodies
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