![Western Blot: PSD93 Antibody (S18-30) [NBP2-12912] - analysis of Rat brain membrane lysate showing detection of Chapsyn-110 protein using Mouse Anti-Chapsyn-110 Monoclonal Antibody, Clone S18-30 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Chapsyn-110 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/PSD93-Antibody-S18-30-Western-Blot-NBP2-12912-img0002.jpg "Western Blot: PSD93 Antibody (S18-30) [NBP2-12912] - analysis of Rat brain membrane lysate showing detection of Chapsyn-110 protein using Mouse Anti-Chapsyn-110 Monoclonal Antibody, Clone S18-30 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Chapsyn-110 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | S18-30 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Fusion protein amino acids 1-852 of rat Chapsyn-110 |
| Localization | Membrane, Cell Junction, Synapse, Postsynaptic Cell Membrane, Cell Projection, Axon |
| Marker | post-Synaptic Marker |
| Specificity | Detects approx. 110 kDa. No cross-reactivity against other MAGUK family members, PSD95, SAP97, SAP102, expressed in transfected cells. Weak human detection. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | DLG2 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | 1 ug/mL of Chapsyn-110 Antibody was sufficient for detection of Chapsyn -110 in 10 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.4), 50% Glycerol |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | DLG2 |
![Immunocytochemistry/Immunofluorescence: PSD93 Antibody (S18-30) [NBP2-12912] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Chapsyn-110 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane, Cell Junction, Synapse, Postsynaptic Cell Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/PSD93-Antibody-S18-30-Immunocytochemistry-Immunofluorescence-NBP2-12912-img0003.jpg "Immunocytochemistry/Immunofluorescence: PSD93 Antibody (S18-30) [NBP2-12912] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Chapsyn-110 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Membrane, Cell Junction, Synapse, Postsynaptic Cell Membrane. Magnification: 60X.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")