Novus Biologicals products are now on bio-techne.com

LDLR Antibody (SJ0197)

Images

 
Western Blot: LDLR Antibody (SJ0197) [NBP2-67414] - Analysis of LDL Receptor on MCF-7 lysates using anti-LDL Receptor antibody at 1/1,000 dilution.
Immunocytochemistry/ Immunofluorescence: LDLR Antibody (SJ0197) [NBP2-67414] - Staining LDL Receptor in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised ...read more
Immunohistochemistry-Paraffin: LDLR Antibody (SJ0197) [NBP2-67414] - Analysis of paraffin-embedded human lung tissue using anti-LDL Receptor antibody. Counter stained with hematoxylin.
Flow Cytometry: LDLR Antibody (SJ0197) [NBP2-67414] - Analysis of Hela cells with LDL Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). ...read more
Immunocytochemistry/ Immunofluorescence: LDLR Antibody (SJ0197) [NBP2-67414] - Staining LDL Receptor in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised ...read more
Immunocytochemistry/ Immunofluorescence: LDLR Antibody (SJ0197) [NBP2-67414] - Staining LDL Receptor in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised ...read more
Immunohistochemistry-Paraffin: LDLR Antibody (SJ0197) [NBP2-67414] - Analysis of paraffin-embedded human liver cancer tissue using anti-LDL Receptor antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: LDLR Antibody (SJ0197) [NBP2-67414] - Analysis of paraffin-embedded human liver tissue using anti-LDL Receptor antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
SJ0197
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

LDLR Antibody (SJ0197) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within Human LDLR aa 811-860 / 860. (SwissProt: P01130 Human; SwissProt: P35951 Mouse)
Localization
Lysosome, Golgi apparatus, Cell membrane, Early endosome, Late endosome, clathrin-coated pit.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
LDLR
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for LDLR Antibody (SJ0197)

  • FH
  • FHC
  • LDL R
  • LDL receptor
  • LDLCQ2
  • LDLR
  • low density lipoprotein receptor
  • low-density lipoprotein receptor class A domain-containing protein 3
  • low-density lipoprotein receptor

Background

The low density lipoprotein (LDL) receptor system coordinates the metabolism of cholesterol, an essential component of the plasma membrane of all mammalian cells. Study of this system has led to an enhanced understanding of the cellular basis of cholesterol homeostasis. It has also brought into focus an important mechanism of metabolic regulation--the process of receptor-mediated endocytosis. Data suggest that the juxtamembranous region of the cytoplasmic domain participates in protein:protein interactions that allow the low density lipoprotein receptor to cluster in coated pits. It has been shown that the family of LDL receptors may serve as viral receptors. Endocytosis of the Flaviviridae viruses, hepatitis C virus, GB virus C/hepatitis G virus, and bovine viral diarrheal virus (BVDV) were shown to be mediated by LDL receptors on cultured cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB4124
Species: Hu
Applications: IHC, Simple Western, WB
NB110-60531
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, WB
DPC900
Species: Hu
Applications: ELISA
NBP2-66888
Species: Hu, Mu, Rt
Applications: IHC-P, IP, Simple Western, WB
NB100-64808
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP, WB
AF7197
Species: Hu, Mu
Applications: ICC, IHC, WB
NBP2-37477
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NBP1-54446
Species: Ch, Hu
Applications: ELISA, GS, ICC/IF, IP, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
NBP2-79854
Species: Hu
Applications: CyTOF-ready, Flow, ICC/IF, IHC, IHC-P, PA, WB
NBP1-82820
Species: Hu
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
NBP3-14454
Species: Hu
Applications: IHC, IHC-P
NBP2-52979
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
AF2258
Species: Mu
Applications: IHC, WB
NB600-582
Species: Ca, Ch, SyHa, Ha, Hu, Pm, Mu, Rt
Applications: IHC-Fr, KO, Simple Western, WB
236-EG
Species: Hu
Applications: BA
NBP2-34294
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P

Publications for LDLR Antibody (NBP2-67414) (0)

There are no publications for LDLR Antibody (NBP2-67414).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for LDLR Antibody (NBP2-67414) (0)

There are no reviews for LDLR Antibody (NBP2-67414). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LDLR Antibody (NBP2-67414) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our LDLR Antibody (SJ0197) and receive a gift card or discount.

Bioinformatics

Gene Symbol LDLR