![Immunocytochemistry/Immunofluorescence: Laminin gamma 1 Antibody (A5) - Azide and BSA Free [NBP2-47842] - Bovine frozen skeletal muscle section was fixed in 4% PFA for 5 min and stained with anti-laminin antibody (NBP2-44751) overnight at 4 C and then stained with a goat anti-rat-Alexa Fluor 488 secondary antibody (green) for 1 hour at room temperature. Section was then mounted in a mounting medium containing Dapi (blue). Image from verified customer review.](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-A5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47842-img0001.jpg "Immunocytochemistry/Immunofluorescence: Laminin gamma 1 Antibody (A5) - Azide and BSA Free [NBP2-47842] - Bovine frozen skeletal muscle section was fixed in 4% PFA for 5 min and stained with anti-laminin antibody (NBP2-44751) overnight at 4 C and then stained with a goat anti-rat-Alexa Fluor 488 secondary antibody (green) for 1 hour at room temperature. Section was then mounted in a mounting medium containing Dapi (blue). Image from verified customer review.")
| Reactivity | Hu, Mu, BvSpecies Glossary |
| Applications | Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF |
| Clone | A5 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-44751). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. |
| Immunogen | Murine EHS laminin preparation |
| Localization | Basement membrane |
| Specificity | Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. This monoclonal antibody reacts with laminin B2/1 chain of ~210kDa and does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipitation and immunofluorescence of human skeletal muscle and kidney with laminin chain-specific monoclonal antibodys. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This monoclonal antibody stains uniformly all human and murine basement membranes. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Rat |
| Gene | LAMC1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 210 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (A5) - Azide and BSA Free [NBP2-47842] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Laminin gamma 1 Antibody (A5).](http://images.novusbio.com/fullsize/Laminin-gamma-1-Antibody-A5-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47842-img0002.jpg "Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (A5) - Azide and BSA Free [NBP2-47842] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Laminin gamma 1 Antibody (A5).")
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