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LAG-3 Antibody [Phycoerythrin]

 (21 Publications)
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HEK293 human embryonic kidney cell line transfected with either (A) human LAG-3 or (B) irrelevant transfectants and eGFP was stained with Goat Anti-Human LAG-3 Phycoerythrin-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2319P). Quadrant markers were set based on control antibody staining (Catalog # IC108P, data not shown). View our protocol for Staining Membrane-associated Proteins.
HEK293 human embryonic kidney cell line transfected with either (A) human LAG-3 or (B) irrelevant transfectants and eGFP was stained with Goat Anti-Human LAG-3 Phycoerythrin-conjugated Antigen Affinity-purified ...read more
CD3+ PHA-treated peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Goat Anti-Human LAG-3 PE-conjugated Antigen ...read more
Human CD4+CD25−LAG3+ T cells suppressed antibody production. a Gating strategy of fluorescence-activated cell sorting analysis for CD4+CD25−LAG3+CD45RA− T cells (LAG3+ Tregs) and CD4+CD25+CD127dimCD45RA− T cells ...read more
Effect of abatacept on the CD4+CD25−LAG3+ T-cell subset in vitro. a Naive CD4+ T cells and antigen-presenting cells (APCs) taken from healthy donors were cultured with anti-CD3 antibody in the presence of abatacept, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Phycoerythrin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Allophycocyanin
FAB2319A
Biotin
BAF2319
Fluorescein
FAB2319F
PerCP (Peridinin-chlorophyll Protein Complex)
FAB2319C
Unconjugated
AF2319
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF2319
Carrier Free
AF2319
Carrier Free
FAB2319C
Carrier Free
FAB2319F
Carrier Free
FAB2319A
Carrier Free
AF2319-SP
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LAG-3 Antibody [Phycoerythrin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human LAG-3
Leu23-Leu450
Accession # P18627
Specificity
Detects human LAG-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse LAG-3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LAG3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
FAB2319P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LAG-3 Antibody [Phycoerythrin]

  • CD223 antigen
  • CD223
  • LAG3
  • LAG-3
  • lymphocyte activating 3
  • lymphocyte activation gene 3 protein
  • lymphocyte-activation gene 3
  • Secreted lymphocyte activation gene 3 protein
  • sLAG-3

Background

LAG-3 (Lymphocyte activation gene-3), also known as CD223, is a member of the immunoglobulin superfamily (IgSF). The mature LAG-3 protein is a 496 amino acid (aa) membrane protein with a 421 aa extracellular region which contains four IgSF domains, a 21 aa transmembrane region and a 54 aa cytoplasmic region. LAG-3 and CD4 molecules share < 20% aa sequence homology but have a similar structure (1, 2). Both molecules bind to MHC class II. LAG-3 binds to MHC class II with higher affinity compared to CD4. Both LAG-3 and CD4 genes are located on the distal part of the short arm of chromosome 12.

LAG-3 is an activation-induced molecule, expressed on activated T cells and NK cells, but not on resting T cells. Studies using LAG-3 -/- mice have shown significant delay of T cell apoptosis following antigen stimulation and increased size of memory T cells pool following infection (3, 4). It also has been reported that anti-LAG-3 antibodies up-regulate T cell activation by blocking interaction of LAG-3 and MHC class II. The study has demonstrated that LAG-3 is selectively expressed on activated CD4+CD25+ TReg cells and plays a role in their suppressive activity (5). This evidence indicated, unlike the interaction of CD4 with MHC class II that plays a positive role in T cell activation, LAG-3 binds to MHC class II and negatively regulates T cell activation through LAG-3 signaling. On the other hand, studies have shown that binding of LAG-3 to MHC class II molecules on antigen presenting cells induce maturation of dendritic cells and cytokine secretion by monocytes through MHC class II signal transduction (6). Taken together, LAG-3 may have two major functions, it negatively regulates T cells activation through LAG-3 signaling and stimulates antigen presenting cells which express MHC class II.

  1. Triebel, F. et al. (1990) J. Exp. Med. 171:1393.
  2. Baixeras, E. et al. (1992) J. Exp. Med 176:327.
  3. Workman, C.J. and D.A. Vignali (2003) Eur. J. Immunol. 33:970.
  4. Workman, C.J. et al. (2004) J. Immunol. 172:5450.
  5. Huang, C.T. et al. (2004) Immunity 21:503.
  6. Andreae, S. et al. (2003) Blood 102:2130.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LAG-3 Antibody (FAB2319P)(21)

We have publications tested in 4 confirmed species: Human, Mouse, Rhesus Macaca (Macaque), Xenograft.

We have publications tested in 2 applications: Bioassay, Flow Cytometry.


Filter By Application
Bioassay
(1)
Flow Cytometry
(11)
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Human
(9)
Mouse
(2)
Rhesus Macaca (Macaque)
(1)
Xenograft
(1)
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Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using FAB2319P Applications Species
Bruss, C;Kellner, K;Albert, V;Hutchinson, JA;Seitz, S;Ortmann, O;Brockhoff, G;Wege, AK; Immune Checkpoint Profiling in Humanized Breast Cancer Mice Revealed Cell-Specific LAG-3/PD-1/TIM-3 Co-Expression and Elevated PD-1/TIM-3 Secretion Cancers 2023-05-04 [PMID: 37174080] (Flow Cytometry, Mouse, Xenograft) Flow Cytometry Mouse, Xenograft
Thomas Kimman, Anne Slomp, Anne Martens, Sarah Grabherr, Shuang Li, Eline van Diest et al. Serpin B9 controls tumor cell killing by CAR T cells Journal for ImmunoTherapy of Cancer 3/17/2023 [PMID: 36931661]
N Hebbar, R Epperly, A Vaidya, U Thanekar, SE Moore, M Umeda, J Ma, SL Patil, D Langfitt, S Huang, C Cheng, JM Klco, S Gottschalk, MP Velasquez CAR T cells redirected to cell surface GRP78 display robust anti-acute myeloid leukemia activity and do not target hematopoietic progenitor cells Nature Communications, 2022-01-31;13(1):587. 2022-01-31 [PMID: 35102167] (Flow Cytometry, Human) Flow Cytometry Human
Julia L. Nugent, Amar Singh, Keith M. Wirth, Scott Hunter Oppler, Laura Hocum Stone, Jody L. Janecek et al. A nonhuman primate model of vertical sleeve gastrectomy facilitates mechanistic and translational research in human obesity iScience 12/17/2021 [PMID: 34877488]
DK Singh, B Singh, SR Ganatra, M Gazi, J Cole, R Thippeshap, KJ Alfson, E Clemmons, O Gonzalez, R Escobedo, TH Lee, A Chatterjee, Y Goez-Gazi, R Sharan, M Gough, C Alvarez, A Blakley, J Ferdin, C Bartley, H Staples, L Parodi, J Callery, A Mannino, B Klaffke, P Escareno, RN Platt, V Hodara, J Scordo, S Gautam, AG Vilanova, A Olmo-Fonta, A Schami, A Oyejide, DK Ajithdoss, R Copin, A Baum, C Kyratsous, X Alvarez, M Ahmed, B Rosa, A Goodroe, J Dutton, S Hall-Urson, PA Frost, AK Voges, CN Ross, K Sayers, C Chen, C Hallam, SA Khader, M Mitreva, TJC Anderson, L Martinez-S, JL Patterson, J Turner, JB Torrelles, EJ Dick, K Brasky, LS Schlesinge, LD Giavedoni, R Carrion, D Kaushal Responses to acute infection with SARS-CoV-2 in the lungs of rhesus macaques, baboons and marmosets Nature Microbiology, 2020-12-18;6(1):73-86. 2020-12-18 [PMID: 33340034] (Flow Cytometry, Rhesus Macaca (Macaque)) Flow Cytometry Rhesus Macaca (Macaque)
Yukari Kobayashi, Daisuke Yamada, Taketo Kawai, Yusuke Sato, Taro Teshima, Yuta Yamada et al. Different immunological effects of the molecular targeted agents sunitinib, everolimus and temsirolimus in patients with renal cell carcinoma International Journal of Oncology 2/4/2020 [PMID: 32319571]
Guro Kristin Melve, Elisabeth Ersvaer, Geir Egil Eide, Einar K. Kristoffersen, Øystein Bruserud Peripheral Blood Stem Cell Mobilization in Healthy Donors by Granulocyte Colony-Stimulating Factor Causes Preferential Mobilization of Lymphocyte Subsets Frontiers in Immunology 5/2/2018 [PMID: 29770133]
Richard M. Powell, David Lissauer, Jennifer Tamblyn, Andrew Beggs, Philip Cox, Paul Moss et al. Decidual T cells exhibit a highly differentiated phenotype and demonstrate potential fetal-specificity and a strong transcriptional response to interferon The Journal of Immunology 11/15/2017 [PMID: 28986438]
S Nakachi, S Sumitomo, Y Tsuchida, H Tsuchiya, M Kono, R Kato, K Sakurai, N Hanata, Y Nagafuchi, S Tateishi, H Kanda, T Okamura, K Yamamoto, K Fujio Interleukin-10-producing LAG3(+) regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis Arthritis Res. Ther., 2017-05-16;19(1):97. 2017-05-16 [PMID: 28511719] (Flow Cytometry, Human) Flow Cytometry Human
RY Huang, A Francois, AR McGray, A Miliotto, K Odunsi Compensatory upregulation of PD-1, LAG-3, and CTLA-4 limits the efficacy of single-agent checkpoint blockade in metastatic ovarian cancer Oncoimmunology, 2016-10-28;6(1):e1249561. 2016-10-28 [PMID: 28197366] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Show All 21 Publications.

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Product General Protocols

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FAQs for LAG-3 Antibody (FAB2319P). (Showing 1 - 2 of 2 FAQs).

  1. Can this LAG-3 antibody be used in ELISA? 
    • We have not validated this particular LAG-3 antibody for ELISA in mouse; however, it has been cited as being used as a detection antibody in a publicatoin (PMID 15946792).
  2. I'm using your LAG-3 antibody in western blot but the observed band is a different size than the predicted band size, do you know what the bands are?
    • There are several alternative splice varients of LAG-3 that could explain the different sized proteins stained by the LAG-3 antibody. It is also believed that there are two secreted forms of LAG-3, one that is about 36 kDa and another at 52 kDa.

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Bioinformatics

Gene Symbol LAG3
Uniprot