L1CAM Antibody (84321) [Unconjugated]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

L1CAM (Neural cell adhesion molecule L1, also known as L1, CD171 and NCAM-L1) is a 200-230 kDa member of the L1 family, Immunoglobulin (Ig) superfamily of molecules. L1 is recognized to play a key role in cell migration, adhesion, neurite outgrowth, myelination and neuronal differentiation. It does so through a series of cis and trans interactions that involve multiple copartners and target receptors. L1 is described as forming both homotypic and heterotypic complexes, the latter with molecules as diverse as the EGFR, NCAM, CD24, neurocan and various alpha _v plus beta ₁ and beta ₃ integrins. Cells known to express L1 include immature oligodendrocytes, CD4+ T cells, B cells and monocytes, premyelinating Schwann cells, intestinal epithelial progenitor cells, and cerebellar granule plus Purkinje cells. Mature human L1 is a 1238 amino acid (aa) type I transmembrane protein. It contains an 1101 aa extracellular region (aa 20-1120) plus a 114 aa cytoplasmic domain (aa 1144-1257). The extracellular region possesses six C2-type Ig-like domains (aa 35-607) followed by five fibronectin (FN) type III repeats (aa 612-1108). The cytoplasmic tail contains no kinase motifs, but does possess a FIGQY peptide that interacts with ankyrin, and an RSLE sequence that mediates clathrin-associated endocytosis. At least five Ser residues are known to be phosphorylated. There are two splice variants, one each in the intracellular and extracellular domain. A deletion of RSLE (aa 1177-1180) adversely affects endocytosis, while a Leu substitution for aa 26-31 interfers with numerous heterotypic interactions. In general, the full-length L1 molecule is a neuron-associated isoform. L1 is known to undergo proteolysis, either by plasmin or ADAMs. This generates soluble isoforms of varying sizes (140-200 kDa) that retain bioactivity, and which can be incorporated into the surrounding ECM. The membrane fragments (30-80 kDa) undergo further processing, most importantly by gamma -secretase, to generate a soluble 28 kDa intracellular domain. This domain is SUMOylated, and believed to possess an NLS at Lys1147. Upon presumed entry into the nucleus, L1 is posited to activate L1-responsive genes. In the extracellular region, human and mouse L1 share 86% aa sequence identity.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 3 confirmed species: Human, Mouse, Transgenic Mouse.

We have publications tested in 4 applications: ICC, IHC, IHC-Fr, Western Blot.

Showing Publications 1 - 5 of 5.

Publications using MAB777	Applications	Species
S Chen, Q Jiang, P Huang, C Hu, H Shen, M Schachner, W Zhao The L1 cell adhesion molecule affects protein kinase D1 activity in the cerebral cortex in a mouse model of Alzheimer's disease Brain Res. Bull., 2020-06-12;162(0):141-150. 2020-06-12 [PMID: 32540419] (Western Blot, Transgenic Mouse)	Western Blot	Transgenic Mouse
SX Chen, JH He, YJ Mi, HF Shen, M Schachner, WJ Zhao A mimetic peptide of &alpha2,6-sialyllactose promotes neuritogenesis Neural Regen Res, 2020-06-01;15(6):1058-1065. 2020-06-01 [PMID: 31823885] (ICC, Human)	ICC	Human
Q Jiang, Q Xie, C Hu, Z Yang, P Huang, H Shen, M Schachner, W Zhao Glioma malignancy is linked to interdependent and inverse AMOG and L1 adhesion molecule expression BMC Cancer, 2019-09-12;19(1):911. 2019-09-12 [PMID: 31510944] (ICC, Western Blot, Human)	ICC, Western Blot	Human
J Xu, C Hu, Q Jiang, H Pan, H Shen, M Schachner The small molecule mimetic agonist trimebutine of adhesion molecule L1 contributes to functional recovery after spinal cord injury in mice Dis Model Mech, 2017-07-14;0(0):. 2017-07-14 [PMID: 28714852] (IHC, Western Blot, Mouse)	IHC, Western Blot	Mouse
Liu Y, Yu Y, Schachner M, Zhao W Neuregulin 1-beta regulates cell adhesion molecule L1 expression in the cortex and hippocampus of mice. Biochem Biophys Res Commun, 2013-10-16;441(1):7-12. 2013-10-16 [PMID: 24140408] (IHC-Fr, Western Blot, Mouse)	IHC-Fr, Western Blot	Mouse

Array MAB777

• L1CAM Antibodies
• L1CAM Antibody Pair
• L1CAM ELISA Kit
• L1CAM Lysate
• L1CAM Proteins
• L1CAM Proteins and Enzymes