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KIST Antibody (OTI2H4)

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Western Blot: KIST Antibody (2H4) [NBP2-03027] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KIST (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KIST.
Western Blot: KIST Antibody (2H4) [NBP2-03027] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KIST (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: KIST Antibody (OTI2H4) [NBP2-03027] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY KIST.
Immunohistochemistry-Paraffin: KIST Antibody (2H4) [NBP2-03027] - Staining of paraffin-embedded Human liver tissue using anti-KIST mouse monoclonal antibody.
Flow Cytometry: KIST Antibody (2H4) [NBP2-03027] - Analysis of Hela cells, using anti-KIST antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Western Blot: KIST Antibody (2H4) [NBP2-03027] Analysis of extracts (35ug) from 9 different cell lines by using anti-KIST monoclonal antibody.
Immunohistochemistry-Paraffin: KIST Antibody (2H4) [NBP2-03027] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-KIST mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI2H4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.82 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72383AF350
Alexa Fluor 405
NBP2-72383AF405
Alexa Fluor 488
NBP2-72383AF488
Alexa Fluor 532
NBP2-72383AF532
Alexa Fluor 594
NBP2-72383AF594
Alexa Fluor 647
NBP2-72383AF647
Alexa Fluor 700
NBP2-72383AF700
Alexa Fluor 750
NBP2-72383AF750
Allophycocyanin
NBP2-72383APC
Allophycocyanin/Cy7
NBP2-72383APCCY7
Biotin
NBP2-72383B
CoraFluor 1
NBP2-72383CL1
DyLight 350
NBP2-72383UV
DyLight 405
NBP2-72383V
DyLight 488
NBP2-72383G
DyLight 550
NBP2-72383R
DyLight 594
NBP2-72383DL594
DyLight 650
NBP2-72383C
DyLight 680
NBP2-72383FR
DyLight 755
NBP2-72383IR
FITC
NBP2-72383F
HRP
NBP2-72383H
Janelia Fluor 525
NBP2-72383JF525
Janelia Fluor 549
NBP2-72383JF549
Janelia Fluor 585
NBP2-72383JF585
Janelia Fluor 635
NBP2-72383JF635
Janelia Fluor 646
NBP2-72383JF646
Janelia Fluor 669
NBP2-72383JF669
PE
NBP2-72383PE
PE/Atto594
NBP2-72383PEATT594
PE/Cy5.5
NBP2-72383PECY55
PE/Cy7
NBP2-72383PECY7
PerCP
NBP2-72383PCP
mFluor Violet 450 SE
NBP2-72383MFV450
mFluor Violet 500 SE
NBP2-72383MFV500
mFluor Violet 610 SE
NBP2-72383MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-72383
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KIST Antibody (OTI2H4) Summary

Immunogen
Full length human recombinant protein of human UHMK1 (NP_787062) produced in HEK293T cell.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
UHMK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
46.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.82 mg/ml
Purity
Immunogen affinity purified

Alternate Names for KIST Antibody (OTI2H4)

  • DKFZp434C1613
  • EC 2.7.11.1
  • FLJ23015
  • kinase interacting with leukemia-associated gene (stathmin)
  • Kinase interacting with stathmin
  • KISKIS protein kinase
  • KIST
  • serine/threonine-protein kinase Kist
  • U2AF homology motif (UHM) kinase 1
  • U2AF homology motif kinase 1

Background

The gene encodes a serine/threonine protein kinase that promotes cell cycle progression through G1 by phosphorylation of the cyclin-dependent kinase inhibitor 1B (p27Kip1), which causes nuclear export and degradation. The encoded protein is also thought to function in the adult nervous system and the gene has been associated with schizophrenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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KIST Antibody
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NBP2-03027
KIST Antibody (OTI2H4)
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC

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Bioinformatics

Gene Symbol UHMK1
Entrez