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KIF22 Antibody

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Western Blot: KIF22 Antibody [NBP2-17053] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KIF22 antibody [N3C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: KIF22 Antibody [NBP2-17053] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with KIF22 antibody [N3C2], Internal diluted at 1:1000. The HRP-conjugated ...read more
Immunocytochemistry/ Immunofluorescence: KIF22 Antibody [NBP2-17053] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KIF22 protein stained by KIF22 antibody, Internal diluted at 1:500. ...read more
Immunohistochemistry-Paraffin: KIF22 Antibody [NBP2-17053] - Analysis of paraffin-embedded RT2 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: KIF22 Antibody [NBP2-17053] - Analysis of paraffin-embedded Hela xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Rt, Po, Bv, PmSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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KIF22 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human KIF22. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Porcine (93%), Chimpanzee (99%), Bovine (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KIF22
Purity
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (88%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for KIF22 Antibody

  • Kid
  • KIDA-328A3.2
  • kinesin family member 22
  • kinesin-like 4
  • Kinesin-like DNA-binding protein
  • Kinesin-like protein 4
  • kinesin-like protein KIF22
  • KNSL4kinesin-like DNA-binding protein pseudogene
  • OBP
  • OBP-1
  • OBP-2
  • origin of plasmid DNA replication-binding protein
  • oriP binding protein

Background

The protein encoded by this gene is a member of kinesin-like protein family. This family of proteins are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. The C-terminal half of this protein has been shown to bind DNA. Studies with the Xenopus homolog suggests its essential role in metaphase chromosome alignment and maintenance. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol KIF22
Uniprot