Kallikrein 3/PSA Antibody (5A11E9) - BSA Free Summary
Immunogen |
Purified recombinant fragment of Prostate Specific Antigen (amino acids 26-251) expressed in E. coli. [UniProt# P07288] |
Localization |
Secreted. |
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
KLK3 |
Purity |
Protein G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- ELISA 1:10000
- Flow Cytometry 1:200-1:400
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Paraffin 1:200-1:1000
- Western Blot 1:500-1:2000
|
Application Notes |
This Prostate Specific Antigen (5A11E9) antibody is useful for Western blot, Flow Cytometry, Immunohistochemistry on paraffin-embedded sections and ELISA. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS |
Preservative |
0.02% Sodium Azide |
Concentration |
1.0 mg/ml |
Purity |
Protein G purified |
Alternate Names for Kallikrein 3/PSA Antibody (5A11E9) - BSA Free
Background
Prostate Specific Antigen (PSA, Kallikrein-3, KLK3) is a serine protease that is usually secreted from the prostate gland and presented in seminal plasma. Prostate Specific Antigen functions in the liquefaction of seminal coagulum, through hydrolysis of the high molecular mass seminal vesicle protein. Its substrates includes semenogelin I & II, fibronectin, insulin-like growth factor binding protein 3 (IGBP3), and laminin. Highly expressed by normal and malignant prostate cancer cells, Prostate Specific Antigen has been implicated in the growth, invasion, and metastasis of prostate cancer cells. Therefore, Prostate Specific Antigen is widely used as a diagnostic marker for prostate cancer.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for Kallikrein 3/PSA Antibody (NBP1-51541). (Showing 1 - 1 of 1 FAQ).
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I am looking for a pair of antibodies against TOTAL PSA to be used in a sandwich immunoassay. I saw that you carry a biotiny-Ab (NBP1-44951). I was wondering if it can be used to detect TOTAL PSA and if you recommend a matched pair?
- A list of our PSA antibodies suitable for use in ELISA can be found using this link. None have been paired specifically with NBP1-44951 but any monoclonal on this list should suffice. I would recommend NBP1-51541 as it is our best selling antibody to this target and should be compatible with NBP1-44951.
Secondary Antibodies
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Isotype Controls
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