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Islet-1 Antibody (1G9Q8)

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Western Blot: Islet-1 Antibody (1G9Q8) [NBP3-15315] - Western blot analysis of extracts of various cell lines, using Islet-1 Rabbit mAb (NBP3-15315) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western Blot: Islet-1 Antibody (1G9Q8) [NBP3-15315] - Western blot analysis of extracts of various cell lines, using Islet-1 Rabbit mAb (NBP3-15315) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ...read more
Immunohistochemistry-Paraffin: Islet-1 Antibody (1G9Q8) [NBP3-15315] - Immunohistochemistry of paraffin-embedded mouse fetal Heart using Islet-1 Rabbit mAb (NBP3-15315) at dilution of 1:100 (40x lens).Perform high ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
1G9Q8
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

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Islet-1 Antibody (1G9Q8) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Islet-1 (P61371). YAANPRPDALMKEQLVEMTGLSPRVIRVWFQNKRCKDKKRSIMMKQLQQQQPNDKTNIQGMTGTPMVAASPERHDGGLQANPVEVQSYQPPWKVLSDFALQ
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
ISL1
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Islet-1 Antibody (1G9Q8)

  • insulin gene enhancer protein ISL-1
  • ISL LIM homeobox 1
  • ISL1 transcription factor, LIM/homeodomain
  • ISL1 transcription factor, LIM/homeodomain, (islet-1)
  • ISL1
  • Isl-1
  • Islet1
  • Islet-1

Background

Islet 1 encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol ISL1