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integrin beta 4 binding protein Antibody

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Western Blot: integrin beta 4 binding protein Antibody [NBP2-16975] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with EIF6 antibody [N1C3-2] diluted at 1:5000. The ...read more
Immunohistochemistry-Paraffin: integrin beta 4 binding protein Antibody [NBP2-16975] - Human colon. EIF6 antibody [N1C3-2] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: integrin beta 4 binding protein Antibody [NBP2-16975] - Various whole cell extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with integrin beta 4 binding protein antibody ...read more
Western Blot: integrin beta 4 binding protein Antibody [NBP2-16975] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with integrin beta 4 binding protein antibody [N1C3-2] ...read more
Genetic Strategies: Western Blot: integrin beta 4 binding protein Antibody [NBP2-16975] - Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Mu, Rt, Po, Bv, Ch, Sh, Xp, ZeSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

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integrin beta 4 binding protein Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human integrin beta 4 binding protein. The exact sequence is proprietary.
Localization
Cytoplasm; Nucleus
Predicted Species
Mouse (99%), Rat (99%), Porcine (98%), Bovine (97%), Zebrafish (92%), Chicken (94%), Sheep (99%), Xenopus (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EIF6
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Drosophila (82%), , Xenopus laevis (91%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for integrin beta 4 binding protein Antibody

  • B(2)GCN homolog
  • b(2)gcn
  • B4 integrin interactor
  • CAB
  • EIF3Ap27(BBP)
  • eIF-6
  • eukaryotic translation initiation factor 3A
  • eukaryotic translation initiation factor 6
  • ITGB4BPintegrin beta 4 binding protein
  • p27 beta-4 integrin-binding protein
  • p27BBP

Background

Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol EIF6
Uniprot