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Integrin beta 1/CD29 Antibody (P4C10) [Alexa Fluor® 488]

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Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [Alexa Fluor® 488] [NBP2-36561AF488] - A surface stain was performed on HeLa cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: Integrin beta 1/CD29 Antibody (P4C10) [Alexa Fluor® 488] [NBP2-36561AF488] - A surface stain was performed on HeLa cells with Integrin beta 1/CD29 [P4C10] Antibody NBP2-36561AF488 (blue) and a ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IP, B/N
Clone
P4C10
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-36561AF350
Alexa Fluor 405
NBP2-36561AF405
Alexa Fluor 532
NBP2-36561AF532
Alexa Fluor 594
NBP2-36561AF594
Alexa Fluor 647
NBP2-36561AF647
Alexa Fluor 700
NBP2-36561AF700
Alexa Fluor 750
NBP2-36561AF750
Allophycocyanin
NBP2-36561APC
Allophycocyanin/Cy7
NBP2-36561APCCY7
Biotin
NBP2-36561B
DyLight 350
NBP2-36561UV
DyLight 405
NBP2-36561V
DyLight 488
NBP2-36561G
DyLight 550
NBP2-36561R
DyLight 594
NBP2-36561DL594
DyLight 650
NBP2-36561C
DyLight 680
NBP2-36561FR
DyLight 755
NBP2-36561IR
FITC
NBP2-36561F
HRP
NBP2-36561H
Janelia Fluor 549
NBP2-36561JF549
Janelia Fluor 646
NBP2-36561JF646
PE
NBP2-36561PE
PE/Atto594
NBP2-36561PEATT594
PE/Cy5.5
NBP2-36561PECY55
PE/Cy7
NBP2-36561PECY7
PerCP
NBP2-36561PCP
Unconjugated
NBP2-36561
mFluor Violet 450 SE
NBP2-36561MFV450
mFluor Violet 500 SE
NBP2-36561MFV500
mFluor Violet 610 SE
NBP2-36561MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-36561
BSA Free
NBP2-36561SS
BSA Free
NBP2-36561
Azide and BSA Free
NBP2-80818
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Integrin beta 1/CD29 Antibody (P4C10) [Alexa Fluor® 488] Summary

Immunogen
HT1080 cells human fibrosarcoma
Specificity
Epitope is not trypsin sensitive.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITGB1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Block/Neutralize
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32803867). Mouse reactivity reported in scientific literature (PMID: 27550917). .

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Integrin beta 1/CD29 Antibody (P4C10) [Alexa Fluor® 488]

  • CD29 antigen
  • CD29
  • Fibronectin receptor subunit beta
  • FNRBVLAB
  • GPIIA
  • Integrin beta 1
  • integrin beta-1
  • integrin VLA-4 beta subunit
  • integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12)
  • ITGB1
  • MDF2
  • MSK12
  • very late activation protein, beta polypeptide
  • VLA-4 subunit beta
  • VLA-BETA

Background

Integrin beta-1 (ITGB1, CD29, VLA-beta) is the beta subunit found in the integrin families, forming a heterodimer integrin receptor through non-covalent bonding with various integrin alpha subunits. Integrin heterodimer containing Integrin beta-1 binds to various cell surface and extracellular proteins (CD49a-f, CD51) to mediate cell to cell and cell to matrix adhesion (1). Integrin beta-1 plays a critical role in the cell adhesion and recognition in embryogenesis, hemostasis, immune response, tissue repair, metastatic diffusion of tumor cells and development (2, 3, 4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Integrin beta 1/CD29 Antibody (NBP2-36561AF488). (Showing 1 - 1 of 1 FAQ).

  1. I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.
    • I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Secondary Antibodies

 

Isotype Controls

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Integrin Beta 1/CD29 - a cell adhesion and cell signaling protein with diverse functions
Integrins are a large family of trasmembrane proteins involved in cell adhesion and form a link between the intracellular cyskeletal proteins and extracellular matrix proteins. Integrins exist as heterodimers consisting of alpha and beta subunits. ...  Read full blog post.
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Bioinformatics

Gene Symbol ITGB1