I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.

Name: Integrin beta 1/CD29 Antibody (MEM-101A) [PE]
Brand: Novus Biologicals
SKU: NB500-383PE
Price: 469 USD

Question

Accepted Answer

I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, Po, Ca, Mu(-)Species Glossary
Applications	WB, Flow, IP, CyTOF-ready
Clone	MEM-101A
Clonality	Monoclonal
Host	Mouse
Conjugate	PE

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 NB500-383AF350

Alexa Fluor 405 NB500-383AF405

Alexa Fluor 488 NB500-383AF488

Alexa Fluor 532 NB500-383AF532

Alexa Fluor 594 NB500-383AF594

Alexa Fluor 647 NB500-383AF647

Alexa Fluor 700 NB500-383AF700

Alexa Fluor 750 NB500-383AF750

Allophycocyanin NB500-383APC

Allophycocyanin/Cy7 NB500-383APCCY7

Biotin NB110-81701

CoraFluor 1 NB500-383CL1

DyLight 350 NB500-383UV

DyLight 405 NB500-383V

DyLight 488 NB500-383G

DyLight 550 NB500-383R

DyLight 594 NB500-383DL594

DyLight 650 NB500-383C

DyLight 680 NB500-383FR

DyLight 755 NB500-383IR

FITC NB500-384

HRP NB500-383H

Janelia Fluor 525 NB500-383JF525

Janelia Fluor 549 NB500-383JF549

Janelia Fluor 646 NB500-383JF646

Janelia Fluor 669 NB500-383JF669

PE/Atto594 NB500-383PEATT594

PE/Cy5.5 NB500-383PECY55

PE/Cy7 NB500-383PECY7

PerCP NB500-383PCP

Unconjugated NB500-383

mFluor Violet 450 SE NB500-383MFV450

mFluor Violet 500 SE NB500-383MFV500

mFluor Violet 610 SE NB500-383MFV610

Catalog# & Formulation	Size	Price
Standard Formulation NB500-383

BSA Free NB500-383

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB500-383AF350
Alexa Fluor 405	NB500-383AF405
Alexa Fluor 488	NB500-383AF488
Alexa Fluor 532	NB500-383AF532
Alexa Fluor 594	NB500-383AF594
Alexa Fluor 647	NB500-383AF647
Alexa Fluor 700	NB500-383AF700
Alexa Fluor 750	NB500-383AF750
Allophycocyanin	NB500-383APC
Allophycocyanin/Cy7	NB500-383APCCY7
Biotin	NB110-81701
CoraFluor 1	NB500-383CL1
DyLight 350	NB500-383UV
DyLight 405	NB500-383V
DyLight 488	NB500-383G
DyLight 550	NB500-383R
DyLight 594	NB500-383DL594
DyLight 650	NB500-383C
DyLight 680	NB500-383FR
DyLight 755	NB500-383IR
FITC	NB500-384
HRP	NB500-383H
Janelia Fluor 525	NB500-383JF525
Janelia Fluor 549	NB500-383JF549
Janelia Fluor 646	NB500-383JF646
Janelia Fluor 669	NB500-383JF669
PE/Atto594	NB500-383PEATT594
PE/Cy5.5	NB500-383PECY55
PE/Cy7	NB500-383PECY7
PerCP	NB500-383PCP
Unconjugated	NB500-383
mFluor Violet 450 SE	NB500-383MFV450
mFluor Violet 500 SE	NB500-383MFV500
mFluor Violet 610 SE	NB500-383MFV610

Integrin beta 1/CD29 Antibody (MEM-101A) [PE]

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Additional Information	Clone MEM-101A was used by HLDA to establish CD designation.
Immunogen	Raji Burkitt's lymphoma cell line (NM_002211.3).
Marker	Stem Cell Marker
Specificity	The antibody MEM-101A reacts with CD29 (integrin beta1 chain), a 130 kDa single chain type I glycoprotein expressed as a heterodimer (non-covalently associated with the integrin alpha subunits 1-6). CD29 is broadly expressed on majority of hematopoietic and non-hematopoietic cells (leukocytes, platelets, fibroblasts, endothelial cells, epithelial cells and mast cells). HLDA VI; WS Code AS A048
Isotype	IgG1
Clonality	Monoclonal
Host	Mouse
Gene	ITGB1
Purity	Protein A purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C in the dark.
Buffer	PBS
Preservative	0.05% Sodium Azide
Purity	Protein A purified

Publications using NB500-383PE	Applications	Species
Moreira ML, Costa-Pereira C, Alves MLR. Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes. Veterinary Parasitology [PMID: 26995719] (FLOW, Canine) Details: Used the PE form of this antibody.	FLOW	Canine
Chen PW, Luo R, Jian X, Randazzo PA. The Arf6 GTPase-Activating Proteins ARAP2 and ACAP1 Define Distinct Endosomal Compartments that Regulate Integrin a5b1 Traffic. J Biol Chem 2014-09-15 [PMID: 25225293] Details: Using the PE conjugated version of NB500-383, catalog number NB500-383PE.