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IMPDH1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and BaF3 mouse pro-B cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 ...read more
IMP Dehydrogenase 1/IMPDH1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antigen Affinity-purified Polyclonal Antibody ...read more
IMP Dehydrogenase 1/IMPDH1 was detected in immersion fixed C2C12 mouse myoblast cell line using Rabbit Anti-Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8554) at ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for IMP Dehydrogenase 1/IMPDH1 at approximately 56 kDa (as indicated) using ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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IMPDH1 Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf21 derived recombinaint human IMP Dehydrogenase 1/IMPDH1
Met1-Tyr563 (predicted)
Accession # P20839
Specificity
Detects human, rat, and mouse IMP Dehydrogenase 1/IMPDH1 in direct ELISAs and Western blots. In direct ELISAs, less than
5% cross-reactivity with recombinant mouse IMPDH2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IMPDH1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 1:200 dilution
  • Simple Western 2 ug/mL
  • Western Blot 1:1000 dilution

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide.
Preservative
Sodium Azide

Notes

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IMPDH1 Antibody [Unconjugated]

  • DKFZp781N0678
  • EC 1.1.1.205
  • IMP (inosine 5'-monophosphate) dehydrogenase 1
  • IMP (inosine monophosphate) dehydrogenase 1
  • IMP Dehydrogenase 1
  • IMPD 1
  • IMPD
  • IMPD1
  • IMPDH 1
  • IMPDH1
  • IMPDH-I
  • inosine-5'-monophosphate dehydrogenase 1
  • LCA11
  • LCA11RP10
  • retinitis pigmentosa 10 (autosomal dominant)
  • RP10
  • sWSS2608

Background

The enzyme IMPDH1 (Inosine-5'-monophosphate dehydrogenase 1) catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.The enzyme is 514 aminoacids long, with a predicted molecular weight of 55 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for IMPDH1 Antibody (AF8554). (Showing 1 - 1 of 1 FAQ).

  1. Do you have a kit for measuring IMPDH activity?
    • We currently do not have any kits to measure IMPDH activity, however we have a number of IMPDH antibody pairs that can be used for WB/IP and ELISA.
mFluor Violet Conjugated Antibodies

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Bioinformatics

Gene Symbol IMPDH1
Uniprot