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IMP2/IGF2BP2 Antibody

Images

 
Western Blot: IGF2BP2 Antibody [NB100-93408] - HEK293 overexpressing IGF2BP2 and probed with (mock transfection in first lane).
Western Blot: IGF2BP2 Antibody [NB100-93408] - (1ug/ml) staining of HepG2 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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IMP2/IGF2BP2 Antibody Summary

Immunogen
Peptide with sequence C-KQQEQKYPQGVASQR corresponding to C-Terminus according to NP_006539.3, NP_001007226.1.
Specificity
This antibody is expected to recognise both reported isoforms (NP_006539.3 and NP_001007226.1).
Predicted Species
Mouse (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGF2BP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
Application Notes
WB: Approx. 60-70 kDa band observed in HepG2 lysates (calculated MW of 66.1 kDa band according to NP_006539.3). In transfected HEK293 transiently expressing IGF2BP2 a band of approx. 65 kDa is observed. This band is not observed in the non-transfected HEK293.
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
IMP2/IGF2BP2 Overexpression Lysate
Publications
Read Publication using NB100-93408.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IMP2/IGF2BP2 Antibody

  • Hepatocellular carcinoma autoantigen p62
  • IF2B2
  • IGF2BP2
  • IGF-II mRNA-binding protein 2
  • IMP2
  • IMP-2IGF2 mRNA-binding protein 2
  • IMP2VICKZ family member 2
  • insulin-like growth factor 2 mRNA binding protein 2
  • insulin-like growth factor 2 mRNA-binding protein 2
  • p62
  • VICKZ2

Background

This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains several four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IMP2/IGF2BP2 Antibody (NB100-93408)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IGF2BP2
Entrez
Uniprot