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Human IL-5R alpha/CD125 - Ready-To-Use ELISA Kit (Colorimetric)

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ELISA: Human IL-5R alpha/CD125 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39626] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
78.1 - 5000 pg/mL (example only; lot dependent)
Sensitivity
24.6 pg/mL (example only; lot dependent)

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Human IL-5R alpha/CD125 - Ready-To-Use ELISA Kit (Colorimetric) Summary

Description
The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours
Standard Curve Range
78.1 - 5000 pg/mL (example only; lot dependent)
Sensitivity
24.6 pg/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IL5RA

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Solution A
  2. Detection Solution B
  3. Instruction manual
  4. Plate sealer for 96 wells
  5. Pre-coated 96T strip plate
  6. Standard Diluent
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human IL-5R alpha/CD125 - Ready-To-Use ELISA Kit (Colorimetric)

  • CD125 antigen
  • CD125
  • CDw125CD125
  • HSIL5R3
  • IL-5 R alpha
  • IL-5 receptor subunit alpha
  • IL5R alpha
  • IL-5R subunit alpha
  • IL5R
  • IL5RA
  • IL-5Ra
  • IL-5R-alpha
  • interleukin 5 receptor type 3
  • interleukin 5 receptor, alpha
  • interleukin-5 receptor alpha chain
  • interleukin-5 receptor alpha subunit
  • interleukin-5 receptor subunit alpha
  • MGC26560

Background

The protein encoded by the IL5RA gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Six alternatively spliced transcript variants encoding three distinct isoforms have been reported. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol IL5RA