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IL-27 Antibody (355025)

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Mouse splenocytes untreated (open histogram) or treated with 1 μg/mL LPS for 24 hours (filled histogram) were stained with Mouse IL‑27 Monoclonal Antibody (Catalog # MAB18342) or isotype control antibody (Catalog # MAB006, not shown), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Mouse splenocytes untreated (open histogram) or treated with 1 μg/mL LPS for 24 hours (filled histogram) were stained with Mouse IL‑27 Monoclonal Antibody (Catalog # MAB18342) or isotype control antibody (Catalog # ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications CyTOF-ready, Flow
Clone
355025
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC18342U-100UG
Alexa Fluor 405
IC18342V-100UG
Alexa Fluor 488
IC18342G-100UG
Alexa Fluor 532
FAB18342X
Alexa Fluor 594
IC18342T-100UG
Alexa Fluor 647
IC18342R-100UG
Alexa Fluor 700
IC18342N-100UG
Alexa Fluor 750
IC18342S-100UG
Allophycocyanin
FAB18342A
Biotin
FAB18342B
CoraFluor 1
FAB18342CL1
DyLight 350
FAB18342D
DyLight 405
FAB18342E
DyLight 488
FAB18342K
DyLight 550
FAB18342L
DyLight 594
FAB18342M
DyLight 650
FAB18342W
DyLight 680
FAB18342Y
DyLight 755
FAB18342Z
FITC
FAB18342F
HRP
FAB18342H
Janelia Fluor 525
FAB18342JF525
Janelia Fluor 549
FAB18342I
Janelia Fluor 646
FAB18342J
Janelia Fluor 669
FAB18342JF669
PE
FAB18342P
PerCP
FAB18342C
mFluor Violet 450 SE
FAB18342MFV450
mFluor Violet 500 SE
FAB18342MFV500
mFluor Violet 610 SE
FAB18342MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB18342-SP
Carrier Free
IC18342V-100UG
Carrier Free
IC18342U-100UG
Carrier Free
IC18342T-100UG
Carrier Free
IC18342S-100UG
Carrier Free
IC18342R-100UG
Carrier Free
IC18342N-100UG
Carrier Free
IC18342G-100UG
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IL-27 Antibody (355025) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-27 heterodimer
Tyr19-Pro228 (EBI3), Phe29-Ser234 (p28)
Accession # O35228 (EBI3) & Q8K3I6 (p28)
Specificity
Detects mouse IL-27 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse (rm) IL‑27 p28, rmIL‑27 EBI3, recombinant human (rh) IL-27 p28, and rhIL-27 EBI3 is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
IL27
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Publications
Read Publications using
MAB18342 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-27 Antibody (355025)

  • IL-27 p28 subunit
  • IL-27 subunit alpha
  • IL27
  • IL-27
  • IL27A
  • IL-27-A
  • IL27-A
  • IL27p28
  • IL30
  • interleukin 27
  • interleukin 30
  • interleukin-27 subunit alpha
  • MGC71873
  • p28IL-27A

Background

Interleukin 27 (IL-27) is a secreted heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI3 (Epstein-Barr virus‑induced gene 3). IL-27 is expressed by monocytes, endothelial cells and dendritic cells. It binds TCCR/WSX-1 on naïve CD4+ T cells and induces the expression of a functional IL-12 receptor, allowing IL-12-induced Th1 polarization. Human EBI3 is 61% amino acid (aa) identical to mouse EBI3 and includes an 20 aa signal peptide and a 209 aa mature protein with two fibronectin type III domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-27 Antibody (MAB18342)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Flow Cytometry.


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Bioinformatics

Gene Symbol IL27
Uniprot