IL-18/IL-1F4 Antibody

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Interleukin-18 (IL-18), also known as IL-1F4 and IFN-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines and is a key molecule in the innate immune response (1). Rat IL-18 is synthesized as a 24 kDa proprotein that contains a 36 amino acid (aa) propeptide and a 158 aa mature region (2). Under inflammatory conditions, the propeptide is cleaved by Caspase-1 in the cytoplasm to liberate the mature nonglycosylated 18 kDa monomeric IL-18 (3, 4). Mature rat IL-18 shares 91% aa sequence identity with mouse IL-18 and 60-64% aa sequence identity with human, canine, feline, porcine, and rhesus macaque IL-18. IL-18 is secreted by a variety of cell types including macrophages, dendritic cells, and epithelial cells (1, 5). Circulating mature IL-18 is sequestered by soluble IL-18 binding proteins (IL-18 BP) that inhibit IL-18 bioactivity (6). IL-18 interacts with the widely expressed IL-18 R alpha which then recruits the signaling subunit IL-18 R beta (7, 8). The IL-1 family member IL-1F7 also binds to IL-18 R alpha but does not recruit IL-18 R beta or induce signaling (9). IL-1F7 binds IL-18 BP and enhances its neutralizing effect on IL-18 activity (9). IL-18 synergizes with other cytokines to activate NK, Th1, and Th17 cells and to increase the production of IFN-gamma (1, 5, 10-12). IL-18 can also promote Th2 cytokine release which reduces the effectiveness of antiviral responses (13, 14). Increased levels of active IL-18 contribute to the severity of autoimmunity and hypertension, while deficiency of IL-18 results in symptoms of metabolic syndrome (1, 5, 15, 16). In cancer, IL-18 stimulates Th1 and NK cells to target tumor cells, but it can also promote angiogenesis, metastasis, and tumor cell immune evasion (11).

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 6 applications: Binding Assay, ELISA Development, IHC, IHC-P, Neutralization, Western Blot.

Showing Publications 1 - 10 of 10.

Publications using AF521	Applications	Species
Mavrogiannis E, Hagdorn Q, Bazioti V et al. Pirfenidone ameliorates pulmonary arterial pressure and neointimal remodeling in experimental pulmonary arterial hypertension by suppressing NLRP3 inflammasome activation Pulmonary Circulation 6/17/2022 [PMID: 35833096]
J Luo, J Chen, C Yang, J Tan, J Zhao, N Jiang, Y Zhao 6-Gingerol protects against cerebral ischemia/reperfusion injury by inhibiting NLRP3 inflammasome and apoptosis via TRPV1 / FAF1 complex dissociation-mediated autophagy International immunopharmacology, 2021-09-17;100(0):108146. 2021-09-17 [PMID: 34537481] (Western Blot, Rat)	Western Blot	Rat
Qi He, Zhenyu Li, Changchang Meng, Jingxian Wu, Yong Zhao, Jing Zhao Parkin-Dependent Mitophagy is Required for the Inhibition of ATF4 on NLRP3 Inflammasome Activation in Cerebral Ischemia-Reperfusion Injury in Rats Cells 8/14/2019 [PMID: 31416289]
S Kuwahara-O, S Maeda, K Kobayashi, Y Minato, K Tanaka, K Yamanishi, M Hata, W Li, T Hayakawa, K Noguchi, H Okamura, H Yagi Interleukin-18 and its receptor are expressed in gonadotropin-releasing hormone neurons of mouse and rat forebrain Neurosci. Lett., 2017-04-01;0(0):. 2017-04-01 [PMID: 28373090] (IHC, Rat)	IHC	Rat
Andreea Oana Mocanu, Anny Mulya, Hazel Huang, Olivia Dan, Hideharu Shimizu, Esam Batayyah et al. Effect of Roux-en-Y Gastric Bypass on the NLRP3 Inflammasome in Adipose Tissue from Obese Rats PLOS ONE 10/5/2015 [PMID: 26437377]
Guo W, Miyoshi K, Dubner R, Gu M, Li M, Liu J, Yang J, Zou S, Ren K, Noguchi K, Wei F Spinal 5-HT3 receptors mediate descending facilitation and contribute to behavioral hypersensitivity via a reciprocal neuron-glial signaling cascade. Mol Pain, 2014-06-09;10(0):35. 2014-06-09 [PMID: 24913307] (IHC, Rat)	IHC	Rat
Miyoshi K, Obata K, Kondo T, Okamura H, Noguchi K Interleukin-18-mediated microglia/astrocyte interaction in the spinal cord enhances neuropathic pain processing after nerve injury. J. Neurosci., 2008-11-26;28(48):12775-87. 2008-11-26 [PMID: 19036970] (IHC, Neutralization, Rat)	IHC, Neutralization	Rat
Haas CS, Amin MA, Allen BB, Ruth JH, Haines GK, Woods JM, Koch AE Inhibition of angiogenesis by interleukin-4 gene therapy in rat adjuvant-induced arthritis. Arthritis Rheum., 2006-08-01;54(8):2402-14. 2006-08-01 [PMID: 16869003] (Neutralization, Rat)	Neutralization	Rat
Felderhoff-Mueser U, Sifringer M, Polley O, Dzietko M, Leineweber B, Mahler L, Baier M, Bittigau P, Obladen M, Ikonomidou C, Buhrer C Caspase-1-processed interleukins in hyperoxia-induced cell death in the developing brain. Ann. Neurol., 2005-01-01;57(1):50-9. 2005-01-01 [PMID: 15622543] (IHC-P, Western Blot, Rat)	IHC-P, Western Blot	Rat
Jordan JA, Guo RF, Yun EC, Sarma V, Warner RL, Crouch LD, Senaldi G, Ulich TR, Ward PA Role of IL-18 in acute lung inflammation. J. Immunol., 2001-12-15;167(12):7060-8. 2001-12-15 [PMID: 11739527] (Binding Assay, ELISA Development, Rat)	Binding Assay, ELISA Development	Rat

Array AF521

• IL-18/IL-1F4 Antibodies
• IL-18/IL-1F4 Antibody Pairs
• IL-18/IL-1F4 ELISA Kits
• IL-18/IL-1F4 Lysate
• IL-18/IL-1F4 Protein
• IL-18/IL-1F4 ELISAs
• IL-18/IL-1F4 Proteins and Enzymes