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IL-12/IL-35 p35 Antibody (27537) [Fluorescein]

 (5 Publications)
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Human peripheral blood mononuclear cells (PBMCs) either untreated (light orange filled histogram) or treated with LPS (dark orange filled histogram) were stained with Mouse Anti-Human/Mouse IL-12/IL-35 p35 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC2191F) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Human peripheral blood mononuclear cells (PBMCs) either untreated (light orange filled histogram) or treated with LPS (dark orange filled histogram) were stained with Mouse Anti-Human/Mouse IL-12/IL-35 p35 ...read more
Mouse splenocytes either untreated (light orange filled histogram) or treated with LPS (dark orange filled histogram) were stained with Mouse Anti-Human/Mouse IL-12/IL-35 p35 Fluorescein-conjugated Monoclonal Antibody ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Flow
Clone
27537
Clonality
Monoclonal
Host
Mouse
Conjugate
Fluorescein

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC1570U-100UG
Alexa Fluor 405
IC1570V-100UG
Alexa Fluor 488
IC1570G-100UG
Alexa Fluor 532
FAB1570X
Alexa Fluor 594
IC1570T-100UG
Alexa Fluor 647
IC1570R-100UG
Alexa Fluor 700
IC1570N-100UG
Alexa Fluor 750
IC1570S-100UG
Allophycocyanin
IC2191A
Biotin
FAB1570B
CoraFluor 1
FAB1570CL1
DyLight 350
FAB1570D
DyLight 405
FAB1570E
DyLight 488
FAB1570K
DyLight 550
FAB1570L
DyLight 594
FAB1570M
DyLight 650
FAB1570W
DyLight 680
FAB1570Y
DyLight 755
FAB1570Z
HRP
FAB1570H
Janelia Fluor 525
FAB1570JF525
Janelia Fluor 549
FAB1570I
Janelia Fluor 646
FAB1570J
Janelia Fluor 669
FAB1570JF669
PerCP (Peridinin-chlorophyll Protein Complex)
IC2191C
Phycoerythrin
IC2191P
Unconjugated
MAB1570-100
mFluor Violet 450 SE
FAB1570MFV450
mFluor Violet 500 SE
FAB1570MFV500
mFluor Violet 610 SE
FAB1570MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB1570-100
Carrier Free
IC2191C
Carrier Free
IC2191P
Carrier Free
IC2191A
Carrier Free
MAB1570-SP
Carrier Free
MAB1570-100
Carrier Free
MAB1570-500
Carrier Free
IC1570V-100UG
Carrier Free
IC1570U-100UG
Carrier Free
IC1570T-100UG
Carrier Free
IC1570S-100UG
Carrier Free
IC1570R-100UG
Carrier Free
IC1570N-100UG
Carrier Free
IC1570G-100UG
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IL-12/IL-35 p35 Antibody (27537) [Fluorescein] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35
Arg23-Ser219
Accession # P29459
Specificity
Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
IL12A
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
IC2191F in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-12/IL-35 p35 Antibody (27537) [Fluorescein]

  • CLMF p35
  • CLMF
  • cytotoxic lymphocyte maturation factor 1, p35
  • Cytotoxic lymphocyte maturation factor 35 kDa subunit
  • IL-12, subunit p35
  • IL-12/IL-35 p35
  • IL-12A
  • IL35 subunit
  • IL35p35
  • interleukin 12, p35
  • interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
  • interleukin-12 alpha chain
  • interleukin-12 subunit alpha
  • NF cell stimulatory factor chain 1
  • NFSK
  • NK cell stimulatory factor chain 1
  • NKSF1
  • p35

Background

Interleukin 12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3+ regulatory T cells and may function as an inhibitory cytokine to suppress T cell proliferation (2). Human FoxP3+ Tregs do not constitutively express IL-35 (3), but expression may be induced by activated dendritic cells (4).
  1. Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248.
  2. Collison, L.W. et al. (2007) Nature 450:566.
  3. Bardel, E. et al. (2008) J. Immunol. 181:6898.
  4. Seyerl, M. et al.(2010) Eur. J. Immunol. 40:321.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-12/IL-35 p35 Antibody (IC2191F)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


Filter By Application
Flow Cytometry
(5)
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Human
(5)
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Showing Publications 1 - 5 of 5.
Publications using IC2191F Applications Species
B Lymphocytes in Multiple Sclerosis: Bregs and BTLA/CD272 Expressing-CD19+ Lymphocytes Modulate Disease Severity Sci Rep, 2016-07-14;6(0):29699. 2016-07-14 [PMID: 27412504] (Flow Cytometry, Human) Flow Cytometry Human
Gironi , Maira, Saresella , Marina, Rovaris , Marco, Vaghi , Matilde, Nemni , Raffaell, Clerici , Mario, Grossi , Enzo A novel data mining system points out hidden relationships between immunological markers in multiple sclerosis. Immun Ageing, 2013-01-10;10(1):1. 2013-01-10 [PMID: 23305498] (Flow Cytometry, Human) Flow Cytometry Human
Al-Daghri NM, Al-Attas O, Alokail MS Vitamin D receptor gene polymorphisms and HLA DRB1*04 cosegregation in Saudi type 2 diabetes patients. J. Immunol., 2012-01-04;188(3):1325-32. 2012-01-04 [PMID: 22219324] (Flow Cytometry, Human) Flow Cytometry Human
Hussain SF, Kong LY, Jordan J, Conrad C, Madden T, Fokt I, Priebe W, Heimberger AB A novel small molecule inhibitor of signal transducers and activators of transcription 3 reverses immune tolerance in malignant glioma patients. Cancer Res., 2007-10-15;67(20):9630-6. 2007-10-15 [PMID: 17942891] (Flow Cytometry, Human) Flow Cytometry Human
Lenz P, Bacot SM, Frazier-Jessen MR, Feldman GM Nucleoporation of dendritic cells: efficient gene transfer by electroporation into human monocyte-derived dendritic cells. FEBS Lett., 2003-03-13;538(1):149-54. 2003-03-13 [PMID: 12633869] (Flow Cytometry, Human) Flow Cytometry Human

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Bioinformatics

Gene Symbol IL12A
Uniprot