IL-12/IL-35 p35 Antibody (27537) [Allophycocyanin]

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

p35 is a 34-36 kDa monomeric member of the IL-6 superfamily of molecules. It is 219 amino acids in length, and possibly contains an N-terminal 22 aa signal sequence. In any event, it does not appear to be individually secreted, and its release from cells is commonly assumed to be as part of a disulfide-linked heterodimer linked to either p40 (forming IL-12) or EBI3 (forming IL-35). Multiple cell types are known to express p35, and include keratinocytes, CD138⁺ plasma cells, naïve B cells, monocyte-derived dendritic cells, macrophages, CD86^lo B7-H1^hi CD206+ B7-DC+ tolerogenic dendritic cells, neutrophils, activated monocytes and macrophages, trophoblasts, testicular endothelial cells, and T cells, including CD4⁺ and CD8⁺ T helper cells, and CD4⁺ CD25⁺ Tregs. The presence of the subunit does not necessarily indicate function, as the p35:p40 heterodimer is associated with promoting inflammatory IFN-gamma production by NK cells, while the p35:EBI3 heterodimer is anti-inflammatory through its inhibition of Th17 cell development. Over aa 23-219, human p35 shares 83% and 57% aa sequence identity with pig and mouse p35, respectively.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: Flow Cytometry.

Showing Publications 1 - 5 of 5.

Publications using IC2191A	Applications	Species
Alvarez, F;Istomine, R;Da Silva Lira Filho, A;Al-Aubodah, TA;Huang, D;Okde, R;Olivier, M;Fritz, JH;Piccirillo, CA; IL-18 is required for the TH1-adaptation of TREG cells and the selective suppression of TH17 responses in acute and chronic infections Mucosal immunology 2023-05-12 [PMID: 37182738] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
X Wu, F Brombacher, ZC Chroneos, CC Norbury, DC Gowda IL-4R&alpha signaling by CD8&alpha+ dendritic cells contributes to cerebral malaria by enhancing inflammatory, Th1, and cytotoxic CD8+ T cell responses The Journal of Biological Chemistry, 2021-03-30;0(0):100615. 2021-03-30 [PMID: 33798555] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
SS Duffy, BA Keating, CJ Perera, JG Lees, RS Tonkin, PGS Makker, P Carrive, O Butovsky, G Moalem-Tay Regulatory T cells and their derived cytokine, interleukin-35, reduce pain in experimental autoimmune encephalomyelitis J. Neurosci., 2019-01-16;0(0):. 2019-01-16 [PMID: 30651334] (Mouse)		Mouse
Wang , Jia M, Shi , Lei, Ma , Cheng J, Ji , Xiao J, Ying , Ruo S, Wu , Xiao Y, Wang , Ke S, Li , Guangyu, Moorman , Jonathan, Yao , Zhi Q Differential regulation of interleukin-12 (IL-12)/IL-23 by Tim-3 drives T(H)17 cell development during hepatitis C virus infection. J Virol, 2013-02-06;87(8):4372-83. 2013-02-06 [PMID: 23388728] (Flow Cytometry, Human)	Flow Cytometry	Human
van Herwijnen M, Wieten L, van der Zee R, van Kooten P, Wagenaar-Hilbers J, Hoek A, den Braber I, Anderton S, Singh M, Meiring H, van Els C, van Eden W, Broere F Regulatory T cells that recognize a ubiquitous stress-inducible self-antigen are long-lived suppressors of autoimmune arthritis. Proc Natl Acad Sci U S A, 2012-08-13;109(35):14134-9. 2012-08-13 [PMID: 22891339] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse

Array IC2191A

• IL-12/IL-35 p35 Antibodies
• IL-12/IL-35 p35 ELISA Kits