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Mouse IgG Isotype Control [Agarose]

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SDS-Page: Mouse IgG Isotype Control [Agarose] [NBP1-97142] - SDS-PAGE of Mouse IgG Whole Molecule Agarose Conjugated . Lane 1: 5 uL Opal Prestained Marker . Lane 2: Reduced Mouse IgG Whole Molecule Agarose Conjugated . Lane 3: Reduced Mouse F(c) Fragment . Lane 4: Reduced Mouse F(ab) Fragment . Lane 5: Mouse IgM Kappa Myeloma Protein . Load: 1 ug per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
SDS-Page: Mouse IgG Isotype Control [Agarose] [NBP1-97142] - SDS-PAGE of Mouse IgG Whole Molecule Agarose Conjugated . Lane 1: 5 uL Opal Prestained Marker . Lane 2: Reduced Mouse IgG Whole Molecule Agarose Conjugated . ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications IP, PAGE
Host
Mouse
Conjugate
Agarose

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alkaline Phosphatase
NBP1-97220
Biotin
NBP1-97078
FITC
NBP1-96789
HRP
NBP1-97073
Rhodamine
NBP1-96790
Texas Red
NBP1-97079
Unconjugated
NBP1-97019-5mg
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Mouse IgG Isotype Control [Agarose] Summary

Description
Store vial at 4C prior to opening. DO NOT FREEZE.

This product is normal Mouse IgG coupled to activated agarose. Assay by immunoelectrophoresis prior to coupling to the beads.
Isotype
IgG
Host
Mouse
Gene
IGHG1
Purity
Multi-step

Applications/Dilutions

Dilutions
  • Immunoprecipitation 1:10 - 1:500
  • SDS-Page 1:100 - 1:2000
Application Notes
This product is suitable for immunoprecipitation and Immunoaffinity purifications.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Multi-step

Alternate Names for Mouse IgG Isotype Control [Agarose]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Publications for IgG Isotype Control (NBP1-97142) (0)

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Product General Protocols

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CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.
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Bioinformatics

Gene Symbol IGHG1