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Mouse IgG Isotype Control [Biotin]

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SDS-Page: Mouse IgG Isotype Control [Biotin] [NBP1-97078] - Lane 1: 5 uL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule Biotin Conjugated. Lane 3: Reduced Mouse F(c) Fragment. Lane 4: Reduced Mouse ...read more
Mouse IgG Isotype Control [Biotin] [NBP1-97078] - Ephrin-A3 immunoreactivity in mouse trigeminal ganglion neurons. Sections were incubated with NBP2-70623B overnight at 4 degrees. Biotinylated mouse IgG was used to help ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications PAGE
Host
Mouse
Conjugate
Biotin
Concentration
LYOPH

Order Details

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Mouse IgG Isotype Control [Biotin] Summary

Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Mouse IgG and anti-Mouse Serum.
Isotype
IgG
Host
Mouse
Gene
IGHG1
Purity
Multi-step

Applications/Dilutions

Dilutions
  • SDS-Page 1:100 - 1:2000
Application Notes
This product can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
Readout System
Reviewed Applications
Read 1 Review rated 5
using
NBP1-97078 in the following application:

Publications
Read Publication using NBP1-97078.

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Multi-step
Reconstitution Instructions
Reconstitute with 1.0 ml deionized water (or equivalent).

Alternate Names for Mouse IgG Isotype Control [Biotin]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Publications for IgG Isotype Control (NBP1-97078)(1)

Review for IgG Isotype Control (NBP1-97078) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using NBP1-97078:
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  5
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Verified Customer
04/26/2023
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Summary

Lot3429

FAQs for IgG Isotype Control (NBP1-97078) (0)

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Additional IgG Products

Research Areas for IgG Isotype Control (NBP1-97078)

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Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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04/26/2023
Application:
Species:

Bioinformatics

Gene Symbol IGHG1