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Goat anti-Rat IgG Secondary Antibody [NL637]

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Detection of human Vimentin by incubating cells with 8 μg/mL of Rat anti-Human Vimentin monoclonal antibody (MAB2105) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Rat IgG NorthernLights™ NL637-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in A172 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).
Detection of human Vimentin by incubating cells with 8 μg/mL of Rat anti-Human Vimentin monoclonal antibody (MAB2105) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Rat IgG ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
NL637

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
BAF005
HRP
HAF005
NL493
NL015
NL557
NL013
Unconjugated
NL013
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NL013
Carrier Free
AF005
Carrier Free
HAF005
Carrier Free
NL015
Carrier Free
NL013
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Goat anti-Rat IgG Secondary Antibody [NL637] Summary

Additional Information
NorthernLights 637 Fluorochrome-labeled Antibody, X Absorbed
Immunogen
Rat IgG heavy and light chains
Specificity
Rat IgG (H+L)
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry
Publications
Read Publication using NL014.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goat anti-Rat IgG Secondary Antibody [NL637]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IGHG1