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Goat anti-Rat IgG Secondary Antibody [NL557]

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Detection of human Vimentin by incubating cells with 8 μg/mL of Rat anti-Human Vimentin monoclonal antibody (MAB2105) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Rat IgG ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
NL557
Suitable Sample Type
23042

Order Details

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Catalog# & Conjugate Size Price
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Goat anti-Rat IgG Secondary Antibody [NL557] Summary

Additional Information
NorthernLights 557 Fluorochrome-labeled Antibody, X Absorbed
Immunogen
Rat IgG heavy and light chains
Specificity
Rat IgG (H+L)
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGHG1
Purity Statement
Antigen Affinity-purified

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry
Publications
Read Publications using
NL013 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goat anti-Rat IgG Secondary Antibody [NL557]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (NL013)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC, IHC, IHC-Fr, IHC-P.


Filter By Application
ICC
(3)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
All Applications
Filter By Species
Human
(4)
Mouse
(2)
All Species
Showing Publications 1 - 6 of 6.
Publications using NL013 Applications Species
JW Opzoomer, JE Anstee, I Dean, EJ Hill, I Bouybayoun, J Caron, T Muliaditan, P Gordon, D Sosnowska, R Nuamah, SE Pinder, T Ng, F Dazzi, S Kordasti, DR Withers, T Lawrence, JN Arnold Macrophages orchestrate the expansion of a proangiogenic perivascular niche during cancer progression Science Advances, 2021-11-03;7(45):eabg9518. 2021-11-03 [PMID: 34730997] (IHC, Mouse) IHC Mouse
I Kladnicka, M Cedikova, J Jedlicka, M Kohoutova, L Muller, I Plavinova, M Kripnerova, M Bludovska, J Kuncova, D Mullerova Chronic DDE Exposure Modifies Mitochondrial Respiration during Differentiation of Human Adipose-Derived Mesenchymal Stem Cells into Mature Adipocytes Biomolecules, 2021-07-21;11(8):. 2021-07-21 [PMID: 34439734] (ICC, Human) ICC Human
G Kaufman, D Skrtic N-Acetyl Cysteine Modulates the Inflammatory and Oxidative Stress Responses of Rescued Growth-Arrested Dental Pulp Microtissues Exposed to TEGDMA in ECM Int J Mol Sci, 2020-10-03;21(19):. 2020-10-03 [PMID: 33023018] (ICC, Human) ICC Human
J Wang, G He, Q Yang, L Bai, B Jian, Q Li, Z Li B-cell lymphoma 2 is associated with advanced tumor grade and clinical stage, and reduced overall survival in young Chinese patients with colorectal carcinoma Oncol Lett, 2018-04-13;15(6):9009-9016. 2018-04-13 [PMID: 29844818] (IHC-P, Human) IHC-P Human
Pettersson U, Walden T, Carlsson P, Jansson L, Phillipson M Female Mice are Protected against High-Fat Diet Induced Metabolic Syndrome and Increase the Regulatory T Cell Population in Adipose Tissue. PLoS ONE, 2012-09-25;7(9):e46057. 2012-09-25 [PMID: 23049932] (IHC-Fr, Mouse) IHC-Fr Mouse
The presence of a side population and its marker ABCG2 in human deciduous dental pulp cells. Biochem. Biophys. Res. Commun., 2010-08-20;400(0):334-9. 2010-08-20 [PMID: 20728430] (ICC, Human) ICC Human

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Product General Protocols

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FAQs for IgG Antibody (NL013) (0)

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Additional IgG Products

Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

Read our latest blog and use the new citation tool on bio-techne.com

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Bioinformatics

Gene Symbol IGHG1