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IgG Antibody (RM104)

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Western Blot: IgG Antibody (RM104) [NBP2-62005] - Western blot of nonreduced(-) and reduced(+) mouse immunoglobulins (20 ng/lane), using NBP2-62005 at 0.2ug/ml. This antibody reacts to nonreduced mouse IgG1, IgG2a, ...read more
ELISA: IgG Antibody (RM104) [Unconjugated] [NBP2-62005] - ELISA of mouse immunoglobulins shows NBP2-62005 reacts to the Fc region of mouse IgG1, IgG2a, IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human or ...read more
ELISA: IgG Antibody (RM104) [Unconjugated] [NBP2-62005] - A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of NBP2-62005 was used as the primary antibody. An ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA
Clone
RM104
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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Catalog# & Conjugate Size Price
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IgG Antibody (RM104) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Mouse IgG
Specificity
This antibody reacts to the Fc region of all subclasses of mouse IgG. No cross reactivity with mouse IgM, IgA, IgE, human IgG, and rat IgG. It may cross react to goat IgG.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
IGHG1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 0.005 ug/ml-0.2 ug/ml
  • Western Blot 0.1 ug/ml-0.5 ug/ml

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for IgG Antibody (RM104)

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for IgG Antibody (NBP2-62005) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for IgG Antibody (NBP2-62005)

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Blogs on IgG.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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Bioinformatics

Gene Symbol IGHG1