![Immunohistochemistry-Paraffin: IgG Antibody (IG266) - IHC-Prediluted [NBP2-45064] - Formalin-fixed, paraffin embedded human Tonsil stained with IgG Monoclonal Antibody (IG266).](http://images.novusbio.com/fullsize/IgG-Antibody-IG266-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-45064-img0001.jpg "Immunohistochemistry-Paraffin: IgG Antibody (IG266) - IHC-Prediluted [NBP2-45064] - Formalin-fixed, paraffin embedded human Tonsil stained with IgG Monoclonal Antibody (IG266).")
| Reactivity | HuSpecies Glossary |
| Applications | IHC |
| Clone | IG266 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | IHC-Prediluted |
| Description | The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining. |
| Immunogen | Purified human IgG |
| Localization | Cytoplasm, Cell Surface and Secreted |
| Marker | B-Cell Marker |
| Specificity | Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This monoclonal antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | IGHG1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Theoretical MW | 75 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for IgG Antibody (NBP2-45064)Find related products by research area.
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CD16 - Find me on macrophages, neutrophils and NK cells CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells.... Read full blog post. |
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Understanding the Importance of Human IgG Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ... Read full blog post. |
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| Gene Symbol | IGHG1 |
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