![Immunocytochemistry/Immunofluorescence: IgG Antibody (IG266) [NBP2-34250] - Multiple myeloma LP-1 and H929 cells stained with NBP2-34250 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/IgG-Antibody-IG266-Immunocytochemistry-Immunofluorescence-NBP2-34250-img0004.jpg "Immunocytochemistry/Immunofluorescence: IgG Antibody (IG266) [NBP2-34250] - Multiple myeloma LP-1 and H929 cells stained with NBP2-34250 antibody. Image from verified customer review.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, MA |
| Clone | IG266 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34648) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. |
| Immunogen | Purified human IgG |
| Localization | Cytoplasm, Cell Surface and Secreted |
| Marker | B-Cell Marker |
| Specificity | Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This monoclonal antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | IGHG1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Theoretical MW | 75 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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reviewed by:
Natalie Erdmann |
ICC | Human | 05/28/2019 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for IgG Antibody (NBP2-34250)Find related products by research area.
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Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp... Read full blog post. |
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CD16 - Find me on macrophages, neutrophils and NK cells CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells.... Read full blog post. |
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Understanding the Importance of Human IgG Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ... Read full blog post. |
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![Immunohistochemistry-Paraffin: IgG Antibody (IG266) [NBP2-34250] - Formalin-fixed, paraffin embedded human Tonsil stained with IgG Monoclonal Antibody (IG266).](http://images.novusbio.com/fullsize/IgG-Antibody-IG266-Immunohistochemistry-Paraffin-NBP2-34250-img0003.jpg "Immunohistochemistry-Paraffin: IgG Antibody (IG266) [NBP2-34250] - Formalin-fixed, paraffin embedded human Tonsil stained with IgG Monoclonal Antibody (IG266).")
![Protein Array: IgG Antibody (IG266) [NBP2-34250] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Anti-IgG Antibody (IG266). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/nbp2-34250_mouse-monoclonal-igg-antibody-ig266-177202495944.jpg "Protein Array: IgG Antibody (IG266) [NBP2-34250] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Anti-IgG Antibody (IG266). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.")
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