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IgD Antibody (IA6-2) [Unconjugated]

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IgD Antibody was detected in immersion fixed paraffin-embedded sections of human Tonsil using Mouse Anti-Human IgD, Monoclonal Antibody (Catalog # MAB9857) at 0.5ug/mL at 37 ° Celsius for 2 minutes. Before incubation ...read more
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (FAB4867P) and either (A) Mouse Anti-Human IgD Monoclonal Antibody (Catalog # MAB9857) or (B) ...read more
IgD was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human IgD Monoclonal Antibody (Catalog # MAB9857) at 5 µg/ml for 1 hour at room temperature followed by incubation with ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, CyTOF-ready, mIF
Clone
IA6-2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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IgD Antibody (IA6-2) [Unconjugated] Summary

Immunogen
Human IgD
Specificity
Detects human IgD in Flow Cytometry.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 3-25 ug/mL
  • Multiplex Immunofluorescence 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IgD Antibody (IA6-2) [Unconjugated]

  • IgD
  • Igh-5
  • Ighd
  • Immunoglobulin D
  • Immunoglobulin Delta

Background

 Immunoglobulin D (IgD) is an antibody isotype typically expressed in the plasma membranes of naïve B cells, usually co-expressed with IgM. IgD is also found secreted in small amounts in serum. Secreted IgD is produced as a monomeric antibody with two heavy chains of the delta class, and two Ig light chains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for IgD Antibody (MAB9857) (0)

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Secondary Antibodies

 

Isotype Controls

Additional IgD Products

Blogs on IgD.

How To Identify B Cell Subsets Using Flow Cytometry
By Victoria OsinskiUsing Flow Cytometry to Identify B Cell SubsetsIdentifying cellular subsets by flow cytometry requires careful and thorough planning in order to ensure the correct subset of cells are identified...  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains ...  Read full blog post.

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