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IFI35 Antibody (OTI1C9)

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Western Blot: IFI35 Antibody (1C9) [NBP2-01108] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFI35 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFI35.
Western Blot: IFI35 Antibody (1C9) [NBP2-01108] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFI35 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunocytochemistry/ Immunofluorescence: IFI35 Antibody (1C9) [NBP2-01108] Staining of COS7 cells transiently transfected by pCMV6-ENTRY IFI35.
Immunohistochemistry-Paraffin: IFI35 Antibody (1C9) [NBP2-01108] - Staining of paraffin-embedded Human pancreas tissue using anti-IFI35 mouse monoclonal antibody.
Flow Cytometry: IFI35 Antibody (1C9) [NBP2-01108] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IFI35 antibody, and then analyzed ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI1C9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.4 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-71004AF350
Alexa Fluor 405
NBP2-71004AF405
Alexa Fluor 488
NBP2-71004AF488
Alexa Fluor 532
NBP2-71004AF532
Alexa Fluor 594
NBP2-71004AF594
Alexa Fluor 647
NBP2-71004AF647
Alexa Fluor 700
NBP2-71004AF700
Alexa Fluor 750
NBP2-71004AF750
Allophycocyanin
NBP2-71004APC
Biotin
NBP2-71004B
CoraFluor 1
NBP2-71004CL1
DyLight 350
NBP2-71004UV
DyLight 405
NBP2-71004V
DyLight 488
NBP2-71004G
DyLight 550
NBP2-71004R
DyLight 594
NBP2-71004DL594
DyLight 650
NBP2-71004C
DyLight 680
NBP2-71004FR
DyLight 755
NBP2-71004IR
FITC
NBP2-71004F
HRP
NBP2-71004H
Janelia Fluor 525
NBP2-71004JF525
Janelia Fluor 549
NBP2-71004JF549
Janelia Fluor 646
NBP2-71004JF646
Janelia Fluor 669
NBP2-71004JF669
PE
NBP2-71004PE
PerCP
NBP2-71004PCP
mFluor Violet 450 SE
NBP2-71004MFV450
mFluor Violet 500 SE
NBP2-71004MFV500
mFluor Violet 610 SE
NBP2-71004MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-71004
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IFI35 Antibody (OTI1C9) Summary

Immunogen
Full length human recombinant protein of human IFI35(NP_005524)produced in HEk293T cell
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
IFI35
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
Theoretical MW
31.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.4 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IFI35 Antibody (OTI1C9)

  • FLJ21753
  • ifi-35
  • IFP 35
  • IFP35interferon-induced 35 kDa protein
  • interferon-induced protein 35

Background

IFI35, also known as Interferon-induced 35 kDa protein, consists of 2 isoforms, a 286 amino acid isoform 1 that is 31.5 kDa and a 188 amino acid isoform that is 32 kDa, has nucleus subcellular location and can be found in a wide range of cell types, including fibroblasts, macrophages, and epithelial cells. Its function is not yet known. Studies of this protein are being performed on research about diverticulitis and ovarian cancer. This protein plays a role in interferon alpha/beta signaling, cytokine signaling in immune system, expression of IFN-induced genes, interferon signaling, and immune system pathways where it interacts with CLEC4G, BATF, PLEKHO1, NMI, and MAPK1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB200-109
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NBP1-76238
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NBP3-09392
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Azide and BSA Free
NBP2-93463
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NBP2-01108
IFI35 Antibody (OTI1C9)
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Bioinformatics

Gene Symbol IFI35
Entrez