![Immunocytochemistry/Immunofluorescence: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Hydrogen/Potassium ATPase beta at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Hydrogen-Potassium-ATPase-Beta-Antibody-2G11-Immunocytochemistry-Immunofluorescence-NB300-583-img0010.jpg "Immunocytochemistry/Immunofluorescence: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Hydrogen/Potassium ATPase beta at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Ca, Ma, RbSpecies Glossary |
| Applications | WB, DB, Flow, ICC/IF, IHC, IP, ChIP |
| Clone | 2G11 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Purified 34 kDa core peptide from deglycosylated hog gastric microsomes. |
| Epitope | This antibody recognizes an epitope between amino acid residues 1-13 or 15-28 located on the cytoplasmic side of the beta-subunit. |
| Specificity | Detects the beta-subunit of hydrogen/potassium ATPase. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ATP4B |
| Purity | Unpurified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | DB usage was reported in scientific literature (PMID: 26686297). |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Ascites diluted with PBS |
| Preservative | 0.05% Sodium Azide |
| Purity | Unpurified |
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Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Both normal and cancer biopsies of deparaffinized Human stomach tissues.](http://images.novusbio.com/fullsize/Hydrogen-Potassium-ATPase-Beta-Antibody-2G11-Immunohistochemistry-Paraffin-NB300-583-img0011.jpg "Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Both normal and cancer biopsies of deparaffinized Human stomach tissues.")
![Immunocytochemistry/Immunofluorescence: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Hydrogen/Potassium ATPase beta at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Hydrogen-Potassium-ATPase-Beta-Antibody-2G11-Immunocytochemistry-Immunofluorescence-NB300-583-img0008.jpg "Immunocytochemistry/Immunofluorescence: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Hydrogen/Potassium ATPase beta at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
![Immunocytochemistry/Immunofluorescence: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Hydrogen/Potassium ATPase beta at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Hydrogen-Potassium-ATPase-Beta-Antibody-2G11-Immunocytochemistry-Immunofluorescence-NB300-583-img0009.jpg "Immunocytochemistry/Immunofluorescence: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Hydrogen/Potassium ATPase beta at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
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![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Both normal and cancer biopsies of deparaffinized Human gastric carcinoma tissues.](http://images.novusbio.com/fullsize/Hydrogen-Potassium-ATPase-Beta-Antibody-2G11-Immunohistochemistry-Paraffin-NB300-583-img0012.jpg "Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Both normal and cancer biopsies of deparaffinized Human gastric carcinoma tissues.")
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