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Human Phospho-Tie-2 DuoSet IC ELISA, 2 Plate

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Human Tie-2 transfected Baf/3 cells (Baf/3-hTie-2) were treated with 600 ng/mL recombinant human Ang-1 for seven minutes to induce tyrosine phosphorylation of Tie-2. Serial dilutions of lysates were analyzed by (A) IP-Western blot and (B) this ELISA. IPs were done using an anti-Tie-2 monoclonal antibody and goat anti-mouse agarose. Immunoblots were incubated with a biotinylated anti-phosphotyrosine monoclonal antibody (Catalog # BAM1676) to detect phospho-Tie-2. Bands were visualized with Streptavidin-HRP (Cat # DY998) followed by chemiluminescent detection using WesternGloTM Chemiluminescent Detection Substrate (Catalog # AR004).
Human Tie-2 transfected Baf/3 cells (Baf/3-hTie-2) were treated with 600 ng/mL recombinant human Ang-1 for seven minutes to induce tyrosine phosphorylation of Tie-2. Serial dilutions of lysates were analyzed by (A) ...read more
Baf/3-hTie-2 cells were untreated or treated with 600 ng/mL recombinant human Ang-1 for seven minutes. ELISA and IP-Western blot (inset) analyses were done using 50 μg and 200 μg of lysate, respectively. IP-Western ...read more
Baf/3-hTie-2 cells were treated with 600 ng/mL recombinant human Ang-1 for seven minutes. The indicated amounts of recombinant extracellular domains of human Tie-2(Catalog #313-TI), human Tie-1 (Catalog #619-TI), human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
Biotin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Biotin
DYC2720-5
Biotin
DYC2720E
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Human Phospho-Tie-2 DuoSet IC ELISA, 2 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
TEK

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Phospho-Tie-2 DuoSet IC ELISA, 2 Plate

  • angiopoietin-1 receptor
  • CD202b antigen
  • CD202b
  • EC 2.7.10
  • EC 2.7.10.1
  • hTIE2
  • p140 TEK
  • soluble TIE2 variant 1
  • soluble TIE2 variant 2
  • TEK tyrosine kinase, endothelial
  • TEK
  • Tie2
  • Tie-2
  • TIE2CD202b
  • Tunica interna endothelial cell kinase
  • Tyrosine-protein kinase receptor TEK
  • Tyrosine-protein kinase receptor TIE-2
  • venous malformations, multiple cutaneous and mucosal
  • VMCM
  • VMCM1

Background

Tie-2, also known as Tek, is a transmembrane receptor tyrosine kinase (RTK) that functions as a receptor for Angiopoietin family proteins. It is expressed by embryonic and adult endothelial cells, hematopoietic stem cells, and a circulating population of proangiogenic monocytes. Tie-2 signaling is activated by Angiopoietins-1 and -4, while it can be either activated or inhibited by Angiopoietins-2 and -3. Tie-2 plays an important role in maintaining vascular integrity by mediating endothelial cell-smooth muscle cell communication and inhibiting endothelial cell apoptosis. It is also required for embryonic development of the endocardium. In addition, Tie-2 signaling mediates the quiescence of bone marrow stem cells in response to osteoblast-produced Angiopoietin-1. This quiescence is critical for maintaining an ongoing hematopoietic capability in the bone marrow.

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Product General Protocols

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Bioinformatics

Gene Symbol TEK