| Reactivity | HuSpecies Glossary |
| Applications | ELISA |
| Conjugate | HRP |
| Background | The Quantikine Human Enolase 2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Enolase 2 in cell culture supernates, cell lysates, serum, and plasma. It contains E. coli-expressed recombinant human Enolase 2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Enolase 2 showed linear curves that were parallel ...to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Enolase 2. Show More |
| Specificity | Natural and recombinant human Enolase 2 |
| Source | N/A |
| Assay Type | Solid Phase Sandwich ELISA |
| Inter-Assay | See PDF Datasheet for details |
| Intra-Assay | See PDF Datasheet for details |
| Spike Recovery | See PDF Datasheet for details |
| Sample Volume | See PDF Datasheet for details |
| Gene | ENO2 |
| Dilutions |
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| Application Notes | No significant interference observed with available related molecules. |
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| Publications |
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| Storage | Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
Enolase 2 (2-phospho-D-glycerate hydrolyase; also Neuron-specific Enolase, NSE, neural enolase and gamma-enolase) is a 46 kDa member of the Enolase family of enzymes. It is expressed in developing neurons and glia, is known to catalyze the generation of phosphoenolpyruvate, and is suggested to possess neurotrophic activity for neurons, likely through an extracellular mechanism. Human Enolase 2 is 434 amino acids (aa) in length. The enzymatic site spans most of the length of the molecule. Enolase 2 exists as both a noncovalently-linked homodimer, or heterodimer with alpha-enolase. Full-length human Enolase 2 shares 99% aa identity with both mouse and canine Enolase 2. It shares 83% aa identity with human enolases 1 and 3.
| Gene Symbol | ENO2 |
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