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Human HspA6 ELISA Kit (Colorimetric)

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ELISA: Human HspA6 ELISA Kit (Colorimetric) [NBP2-62153] - Standard Reference Curve
ELISA: Human HspA6 ELISA Kit (Colorimetric) [NBP2-62153] - Standard Reference Curve

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell lysates, serum, and tissue
Standard Curve Range
0.156 - 10 ng/ml
Sensitivity
62 pg/ml

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Human HspA6 ELISA Kit (Colorimetric) Summary

Standard Curve Range
0.156 - 10 ng/ml
Sensitivity
62 pg/ml
Assay Type
Sandwich ELISA
Inter-Assay
The Inter-Assay Coefficient of variation has been determined to be <10%.
Intra-Assay
The Intra-Assay Coefficient of variation has been determined to be <10%.
Spike Recovery
N/A
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
HSPA6

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. 5X Extraction reagent
  2. Antibody diluent
  3. Antibody
  4. Conjugate diluent
  5. HRP Conjugate
  6. Microtiter plate
  7. Sample diluent
  8. Standard
  9. Stop solution 2
  10. TMB Substrate
  11. Wash buffer concentrate

Alternate Names for Human HspA6 ELISA Kit (Colorimetric)

  • heat shock 70 kDa protein 6
  • Heat shock 70 kDa protein B'
  • heat shock 70kD protein 6 (HSP70B')
  • heat shock 70kDa protein 6 (HSP70B')
  • HSP70B'

Background

HSP70B is a unique member of the human Hsp70 chaperone involved in cellular processes such as protein trafficking, folding, and prevention of aggregation. This protein is strictly stress-inducible, having little or no basal expression levels in most cells. HSP70B and Hsp72 are closely related and play cooperative roles in cell survival of proteotoxic stress. Recombinant human HSP70B, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Human HspA6 ELISA
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NBP2-62153
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Bioinformatics

Gene Symbol HSPA6