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HSP27 Antibody [FITC]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, HaSpecies Glossary
Applications WB, ICC/IF, IHC, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
FITC

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-75477AF350
Alexa Fluor 405
NBP1-75477AF405
Alexa Fluor 488
NBP1-75477AF488
Alexa Fluor 532
NBP1-75477AF532
Alexa Fluor 594
NBP1-75477AF594
Alexa Fluor 647
NBP1-75477AF647
Alexa Fluor 700
NBP1-75477AF700
Alexa Fluor 750
NBP1-75477AF750
Allophycocyanin
NBP1-75477APC
Biotin
NBP1-75477B
CoraFluor 1
NBP1-75477CL1
DyLight 350
NBP1-75477UV
DyLight 405
NBP1-75477V
DyLight 488
NBP1-75477G
DyLight 550
NBP1-75477R
DyLight 594
NBP1-75477DL594
DyLight 650
NBP1-75477C
DyLight 680
NBP1-75477FR
DyLight 755
NBP1-75477IR
HRP
NBP1-75477H
Janelia Fluor 525
NBP1-75477JF525
Janelia Fluor 549
NBP1-75477JF549
Janelia Fluor 585
NBP1-75477JF585
Janelia Fluor 635
NBP1-75477JF635
Janelia Fluor 646
NBP1-75477JF646
Janelia Fluor 669
NBP1-75477JF669
PE
NBP1-75477PE
PerCP
NBP1-75477PCP
Unconjugated
NBP1-75477
mFluor Violet 450 SE
NBP1-75477MFV450
mFluor Violet 500 SE
NBP1-75477MFV500
mFluor Violet 610 SE
NBP1-75477MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-75477
BSA Free
NBP1-75477SS
BSA Free
NBP1-75477
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HSP27 Antibody [FITC] Summary

Immunogen
A synthetic peptide made to an internal region of the human Hsp27 protein (within residues 80-150). [Swiss-Prot# P04792]
Localization
Cytoplasm and nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
Predicted Species
Porcine (94%), Bovine (95%), Hamster (94%), Canine (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPB1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (80%).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for HSP27 Antibody [FITC]

  • 28 kDa heat shock protein
  • DKFZp586P1322
  • Estrogen-regulated 24 kDa protein
  • Heat shock 27 kDa protein
  • heat shock 27kD protein 1
  • heat shock 27kDa protein 1
  • heat shock protein beta-1
  • HMN2B
  • HS.76067
  • HSP25
  • HSP27
  • HSP27HSP 27
  • HSP28CMT2F
  • HSPB1
  • SRP27
  • Stress-responsive protein 27

Background

Heat shock protein 27 is a molecular chaperone involved in protection of cells during cellular stress events. The activity of HSP27 protects from apoptotic events caused by heat shock and other distress. Immunohistochemical detection of HSP27 has been suggested as a qualitative biomarker for colorectal cancer. It is also highly expressed in lung cancer, cerebral inflammation, ischemia-reperfusion injury, and glaucoma. Interestingly, HSP27 overexpression can counteract the activity of multi-drug resistance proteins in breast cancer. Polyphenols downregulate the expression of HSP27, as well as many antioxidant enzymes, resulting in susceptibility to stress and damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HSPB1