Home » HSP27 » HSP27 Antibodies » HSP27 Antibody [Biotin]
Novus Biologicals products are now on bio-techne.com

HSP27 Antibody [Biotin]

 Submit a Review

Images

 
There are currently no images for HSP27 Antibody (NBP1-75477B).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, HaSpecies Glossary
Applications WB, ICC/IF, IHC, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-75477AF350
Alexa Fluor 405
NBP1-75477AF405
Alexa Fluor 488
NBP1-75477AF488
Alexa Fluor 532
NBP1-75477AF532
Alexa Fluor 594
NBP1-75477AF594
Alexa Fluor 647
NBP1-75477AF647
Alexa Fluor 700
NBP1-75477AF700
Alexa Fluor 750
NBP1-75477AF750
Allophycocyanin
NBP1-75477APC
CoraFluor 1
NBP1-75477CL1
DyLight 350
NBP1-75477UV
DyLight 405
NBP1-75477V
DyLight 488
NBP1-75477G
DyLight 550
NBP1-75477R
DyLight 594
NBP1-75477DL594
DyLight 650
NBP1-75477C
DyLight 680
NBP1-75477FR
DyLight 755
NBP1-75477IR
FITC
NBP1-75477F
HRP
NBP1-75477H
Janelia Fluor 525
NBP1-75477JF525
Janelia Fluor 549
NBP1-75477JF549
Janelia Fluor 585
NBP1-75477JF585
Janelia Fluor 635
NBP1-75477JF635
Janelia Fluor 646
NBP1-75477JF646
Janelia Fluor 669
NBP1-75477JF669
PE
NBP1-75477PE
PerCP
NBP1-75477PCP
Unconjugated
NBP1-75477
mFluor Violet 450 SE
NBP1-75477MFV450
mFluor Violet 500 SE
NBP1-75477MFV500
mFluor Violet 610 SE
NBP1-75477MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-75477
BSA Free
NBP1-75477SS
BSA Free
NBP1-75477
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

HSP27 Antibody [Biotin] Summary

Immunogen
A synthetic peptide made to an internal region of the human Hsp27 protein (within residues 80-150). [Swiss-Prot# P04792]
Localization
Cytoplasm and nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
Predicted Species
Porcine (94%), Bovine (95%), Hamster (94%), Canine (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockout Validated
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Readout System

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (80%).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for HSP27 Antibody [Biotin]

  • 28 kDa heat shock protein
  • DKFZp586P1322
  • Estrogen-regulated 24 kDa protein
  • Heat shock 27 kDa protein
  • heat shock 27kD protein 1
  • heat shock 27kDa protein 1
  • heat shock protein beta-1
  • HMN2B
  • HS.76067
  • HSP25
  • HSP27
  • HSP27HSP 27
  • HSP28CMT2F
  • HSPB1
  • SRP27
  • Stress-responsive protein 27

Background

Heat shock protein 27 is a molecular chaperone involved in protection of cells during cellular stress events. The activity of HSP27 protects from apoptotic events caused by heat shock and other distress. Immunohistochemical detection of HSP27 has been suggested as a qualitative biomarker for colorectal cancer. It is also highly expressed in lung cancer, cerebral inflammation, ischemia-reperfusion injury, and glaucoma. Interestingly, HSP27 overexpression can counteract the activity of multi-drug resistance proteins in breast cancer. Polyphenols downregulate the expression of HSP27, as well as many antioxidant enzymes, resulting in susceptibility to stress and damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

HspA4 Antibody
NBP1-81696
Western Blot HspA4 Antibody
Immunohistochemistry-Paraffin HspA4 Antibody
Western Blot HspA4 Antibody
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
HSP60 Antibody -
BSA Free
NBP1-77397
Immunocytochemistry/ Immunofluorescence HSP60 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence HSP60 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence HSP60 Antibody - BSA Free
(4 Reviews)
(6 Publications)
Species: Hu, Mu
Applications: Flow, IB, ICC/IF, IHC, IHC-P, KD, Simple Western, WB
HSP90 alpha
Antibody
NB120-2928
Immunohistochemistry HSP90 alpha Antibody
Immunohistochemistry HSP90 alpha Antibody
Immunohistochemistry-Paraffin HSP90 alpha Antibody
(13 Publications)
Species: Hu, Mu, Pm, Rb, Rt, Sh
Applications: ICC/IF, IHC, IHC-P, IP, WB
ERK2 Antibody
[Unconjugated]
AF1230
Western Blot ERK2 Antibody [Unconjugated]
Immunohistochemistry ERK2 Antibody [Unconjugated]
Knockout Validated ERK2 Antibody [Unconjugated]
(3 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, WB
p38 alpha Antibody
[Unconjugated]
AF8691
Simple Western p38 alpha Antibody [Unconjugated]
Western Blot p38 alpha Antibody [Unconjugated]
Immunohistochemistry p38 alpha Antibody [Unconjugated]
(1 Review)
(11 Publications)
Species: Hu, Mu, Rt
Applications: IHC, KO, Simple Western, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(810 Publications)
Species: Hu
Applications: BA
HSP70/HSPA1A
[Biotin]
DYC1663-2
ELISA HSP70/HSPA1A [Biotin]
ELISA HSP70/HSPA1A [Biotin]
ELISA HSP70/HSPA1A [Biotin]
(25 Publications)
Species: Hu, Mu, Rt
Applications: ELISA
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
SGSM3 Antibody
NBP2-13304
Immunohistochemistry-Paraffin SGSM3 Antibody
Immunocytochemistry/ Immunofluorescence SGSM3 Antibody
Western Blot SGSM3 Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
AlphaB
Crystallin/CRYAB Anti ...
NBP1-47708
Western Blot AlphaB Crystallin/CRYAB Antibody (OTI6A9)
Flow Cytometry AlphaB Crystallin/CRYAB Antibody (OTI6A9)
Immunocytochemistry/ Immunofluorescence AlphaB Crystallin/CRYAB Antibody (OTI6A9)
(1 Review)
(1 Publication)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
HSPA8/HSC71/Hsc70
Antibody (13D3)
NB120-2788
Immunocytochemistry/ Immunofluorescence HSPA8/HSC71/Hsc70 Antibody (13D3)
Immunocytochemistry/ Immunofluorescence HSPA8/HSC71/Hsc70 Antibody (13D3)
Immunocytochemistry/ Immunofluorescence HSPA8/HSC71/Hsc70 Antibody (13D3)
(2 Reviews)
(28 Publications)
Species: Bv, Fe, Fi, Ha, Hu, Mu, Pm, Rt
Applications: B/N, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Simple Western, WB
Caspase-3 Antibody
[Unconjugated] - Acti ...
AF835
Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active
Immunohistochemistry Caspase-3 Antibody [Unconjugated] - Active
Immunocytochemistry Caspase-3 Antibody [Unconjugated] - Active
(7 Reviews)
(386 Publications)
Species: Hu, Mu
Applications: ICC, IHC
POLDIP2 Antibody
NBP1-68874
Western Blot POLDIP2 Antibody
Western Blot POLDIP2 Antibody
(1 Publication)
Species: Hu, Mu, Rt
Applications: PEP-ELISA, WB
Crk Antibody
(3G11E8) - BSA Free
NBP2-37568
ELISA Crk Antibody (3G11E8) - BSA Free
Western Blot Crk Antibody (3G11E8) - BSA Free
Western Blot Crk Antibody (3G11E8) - BSA Free
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
GRAP2 Antibody
(OTI1G2)
NBP2-00764
Flow Cytometry GRAP2 Antibody (OTI1G2)
Flow Cytometry GRAP2 Antibody (OTI1G2)
Western Blot GRAP2 Antibody (OTI1G2)
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
AHSA1 Antibody
(1A2-A8)
H00010598-M01
Western Blot AHSA1 Antibody (1A2-A8)
Sandwich ELISA AHSA1 Antibody (1A2-A8)
(6 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, S-ELISA, WB
AIMP2 Antibody
NBP1-81575
Immunohistochemistry-Paraffin AIMP2 Antibody
Immunohistochemistry-Paraffin AIMP2 Antibody
Immunohistochemistry-Paraffin AIMP2 Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
MAPKAPK2 Antibody
(2B3)
H00009261-M01
Proximity Ligation Assay MAPKAPK2 Antibody (2B3)
Immunocytochemistry/ Immunofluorescence MAPKAPK2 Antibody (2B3)
Immunohistochemistry-Paraffin MAPKAPK2 Antibody (2B3)
(1 Review)
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, IP, PLA, Simple Western, WB

Publications for HSP27 Antibody (NBP1-75477B) (0)

There are no publications for HSP27 Antibody (NBP1-75477B).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HSP27 Antibody (NBP1-75477B) (0)

There are no reviews for HSP27 Antibody (NBP1-75477B). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSP27 Antibody (NBP1-75477B) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional HSP27 Products

Research Areas for HSP27 Antibody (NBP1-75477B)

Find related products by research area.

Blogs on HSP27.

Check out the latest blog posts on HSP27.
Caspase 8 - a key mediator of apoptosis
Programmed cell death via apoptosis is a key controlled physiological process instigated by the cell death receptor family, their ligands, and the caspase cysteine protease family. All caspases exist in a precursor form that contains a prodomain, a...  Read full blog post.
FLICE, FLICE, baby
Cell death via apoptosis is a fundamental cellular function triggered by the cell death receptor family and their ligands which signal through downstream adaptor molecules and the caspase protease family. All caspases exist in a precursor form compose...  Read full blog post.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
TNF-alpha [Unconjugated]
210-TA-005

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HSP27 Antibody [Biotin] and receive a gift card or discount.


Bioinformatics

Gene Symbol HSPB1